Yixin Shi’s research is focused on molecular biology, bacteriology and biochemistry. Shi analyzes the virulent elements necessary for bacterial survival in host environments, homeostasis of metal ions, and two-component regulation systems in Porphyromonas gingivalis.
His goal is to characterize the molecular mechanisms essential for virulent bacteria including Salmonella, Escherichia coli and P. gingivalis. He also investigates post-transcriptional regulation of metal ion homeostasis of bacteria in the conditions that mimic host environments. His work addresses regulatory mechanisms underlying the secretion of multiple virulence factors through a novel secretion system.
Professor Shi received his Ph.D. in bacterial genetics from Kyushu University, Japan, and served as a postdoctoral research associate in the Department of Molecular Microbiology at Washington University School of Medicine, St. Louis. His research currently focuses on gene regulation in P. gingivalis, E. coli andSalmonella.
Education
Ph.D. Bacterial Genetics, Kyushu University, Japan 2000
Natasha Weatherspoon-Griffin, Dezhi Yang, Wei Kong, Zichun Hua, and Yixin Shi. The CpxR/CpxA Two-Component Regulatory System Upregulates the Multidrug Resistance Cascade to Facilitate Escherichia coli Resistance to A Model Antimicrobial Peptide. The Journal of Biological Chemistry (2014).
Yixin Shi (the first and corresponding author), Guang Zhao, and Wei Kong. Genetic analysis of riboswitch-mediated transcriptional regulation responding to Mn2+ in Salmonella. The Journal of Biological Chemistry (2014).
Natasha Weatherspoon-Griffin, Guang Zhao, Wei Kong, Ying Kong, Morigen, Helene Andrews-Polymenis, Michael McClelland, and Yixin Shi. The CpxR/CpxA two-component system upregulates two Tat-dependent peptidoglycan amidases to confer bacterial resistance to antimicrobial peptide. Journal of Biological Chemistry (2011).
Guang Zhao, Natasha Weatherspoon, Wei Kong, Roy Curtiss III, and Yixin Shi. A Dual-Signal Regulatory Circuit Activates Transcription of A Set of Divergent Operons in Salmonella typhimurium. Proceedings of the National Academy of Sciences (2008).
Haowei Song, Wei Kong(contributed equally), Natasha Weatherspoon, Guozheng Qin, William Tyler, John Turk, Roy Curtiss 3rd, and Yixin Shi. Modulation of the regulatory activity of bacterial two-component systems by SlyA. The Journal of Biological Chemistry (2008).
Wei Kong, Natasha Weatherspoon, and Yixin Shi. Molecular Mechanism for Establishment of Signal-dependent Regulation in the PhoP/PhoQ System. The Journal of Biological Chemistry (2008).
Mariko Naito, Eiko Sakai, Yixin Shi, Hiroshi Ideguchi, Mikio Shoji, Naoya Ohara, Kenji Yamamoto, Koji Nakayama. Porphyromonas gingivalis-induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase. Mol Microbiol (2006).
Michael Cromie, Yixin Shi, Tammy Latifi, Eduardo Groisman. An RNA sensor for intracellular Mg2+. Cell (2006).
Hsu Fong-Fu, John Turk, Elizabeth Rhoades, David Russell, Yixin Shi, Eduardo Groisman. Structural Characterization of Cardiolipin by Tandem Quadrupole and Multiple-stage Quadrupole Ion-Trap Mass Spectrometry with Electrospray Ionization. J Am Soc Mass Spectrom (2005).
Hsu Fong-Fu, John Turk, Yixin Shi, Eduardo Groisman. Characterization of acylphosphatidylglycerols from Salmonella typhimurium by tandem mass spectrometry with electrospray ionization. J Am Soc Mass Spectrom (2004).
Yixin Shi, Michael Cromie, Hsu Fong-Fu, John Turk, Eduardo Groisman. PhoP-regulated Salmonella resistance to the antimicrobial peptides magainin 2 and polymyxin B. Molecular Microbiology (2004).
Yixin Shi, Tammy Latifi, Michael Cromie, Eduardo Groisman. Transcriptional control of the antimicrobial peptide resistance ugtL gene by the salmonella PhoP and SlyA regulatory proteins. The Journal of Biological Chemistry (2004).
M Shoji, D Ratnayake, Yixin Shi, T Kadowaki, K Yamamoto, F Yoshimura, A Akamine, M Curtis, K Nakayama. Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis: cell surface polysaccharide as an anchorage for gingipains. Microbiology (2002).
D Atnayake, S Wai, Yixin Shi, K Amako, H Nakayama, K Nakayama. Ferritin from the obligate anaerobe Porphyromonas gingivalis: purification, gene cloning and mutant studies. Microbiology (2000).
W Kong, S Shiota, Yixin Shi, H Nakayama, K Nakayama. A novel peroxiredoxin of the plant Sedum lineare is a homologue of Escherichia coli bacterioferritin co-migratory protein (Bcp). Biochem. J (2000).
Yixin Shi, Wei Kong, Koji Nakayama. Human lactoferrin binds and removes the hemoglobin receptor protein of the periodontopathogen Porphyromonas gingivalis. The Journal of Biololical Chemistry (2000).
Kadowaki, T, Nakayama, K, Okamoto, K, Abe, N, Baba, A, Shi, Yixin, Ratnayake, D, Yamamoto, K. Porphyromonas gingivalis proteinases as virulence determinants in progression of periodontal diseases. (2000).
Research Activity
Shi,Yixin*, Kong,Wei. Study of the PorX-PorY Two-component System in Porphyromonas Gingivalis. HHS-NIH-NIDCR(3/1/2015 - 2/28/2019).
Wang,Xiao*, Shi,Yixin. Mathematics of Synthetic Gene Networks. NSF-MPS-DMS(8/1/2011 - 7/31/2016).
