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Wei Kong

Associate Research Professor
Faculty, TEMPE Campus, Mailcode 7501
Biography

Dr. Wei Kong, research assistant professor, received a Ph.D. degree in bacterial genetics from the Kyushu University, Japan in 2000.  Dr. Kong was a Postdoctoral Associate for the project of development of live bacterial vaccine vector, mentored by Dr. Roy Curtiss III, at the Department of Biology at Washington University in St. Louis and the Biodesign Institute at Arizona State University,

Dr. Kong and others in Dr. Curtiss lab have developed a protective antigen and DNA vaccine delivery platform using self-destructing Salmonella for the prevention and treatment of infectious and non-infectious diseases (http://www.eurekalert.org/pub_releases/2008-07/asu-fft070808.php) (http://www.eurekalert.org/pub_releases/2012-11/asu-ndv110512.php) .

 

Currently, Dr. Kong’s research interests are: 1) with NIH/NCI support, developself-destructing Salmonella based anti-colorectal cancer therapeutic vaccine; 2) with NIH/NIAID support, develop self-destructing Salmonella based anti-Influenza vaccine; 3) develop self-destructing Salmonella based anti-Eimeria vaccine.

Selected Publications

2012

Kong, W, M. Brovold, B. A. Koeneman, J. E. Clark - Curtiss, and R. Curtiss III. Turning self - destructing Salmonella into a universal DNA vaccine delivery platform.  Proc. Natl. Acad. Sci. USA.  109 (47): 19414 - 19419 (Selected as Faculty of 1000 Microbiology). (2012)

2011

Zhao, G, W. Kong (Co - first author), Weatherspoon - Griffin, N., J. E. Clark - Curtiss and Y. Shi. Premature transcription termination requires Mg2  - enhanced MgtL translation within Mg2 riboswitch. EMBO J. 30 (8): 1485 – 1496. (2011)

Ashraf S., W. Kong, S. F. Wang, J. Yang, and R. Curtiss III. Protective cellular responses elicited by vaccination with influenza nucleoprotein delivered by live recombinant attenuated Salmonella  vaccine. Vaccine. 29 (23): 3990 - 4002. (2011)

Weatherspoon-Griffin, N., G. Zhao, W. Kong, Y. Kong, M. Morigen, H. Andrews - Polymenis, M. McClelland, and Y. Shi. The CpxR/CpxA two - component system upregulates two Tat - dependent peptidoglycan amidases to confer bacterial resistance to antimicrobial peptide. J. Biol. Chem. 286 (7): 5529 - 5539. (2011)

Zhang X.M., S. Y. Wanda, K. Brenneman, W. Kong, X. Zhang, K. Roland and R. Curtiss III. Improving Salmonella vector with rec mutation to stabilize the DNA cargoes. BMC Microbiology 11. MS:1038164561476335. (2011)

2010

Wang S.F., Y.H. Li, G. Scarpellini, W. Kong, H.Y. Shi, C.H. Baek, B. Gunn, S.Y. Wanda, K.L. Roland, X. Zhang, P. Senechal-Willis and R. Curtiss III.  Salmonella vaccine vectors displaying delayed antigen synthesis in vivo to enhance immunogenicity. Infect. Immun. 78: 3969 - 3980. (2010)

Ameiss, K., S. Ashraf, W. Kong, A. Pekosz, W.H. Wu, J.N. Billaud, and R. Curtiss III. Delivery of woodchuck hepatitis virus - like particle presented influenza M2e by recombinant attenuated Salmonella displaying a delayed lysis phenotype. Vaccine. 28: 6704 - 6713. (2010)

Curtiss, R. III, W. Xin, Y. Li, W. Kong, S. Y. Wanda, B. M. Gunn, and S. Wang. New technologies in using recombinant attenuated Salmonella vaccine vectors. Crit. Rev. Immunol. 30: 255 - 270. (2010)

2009

Curtiss, R. III, S.Y. Wanda, B. Gunn, X. Zhang, S.A. Tinge, V. Ananthnarayan, H. Mo, S. F. Wang, and W. Kong. Salmonella  vaccine strains with regulated delayed attenuation in vivo. Infect. Immun. 77: 1071 - 1082. (2009)

Zhang, X., W. Kong, S. Ashraf, and R. Curtiss III. A “one-plasmid” system to generate influenza virus in cultured chicken cells for potential use in influenza vaccine. J. Virol. 83: 9296 - 9303. (2009)

2008

Zhao G., N. Weatherspoon, W. Kong, R. Curtiss III, and Y. Shi. A Dual- Signal Regulatory Circuit Activates Transcription of A Set of Divergent Operons in Salmonella typhimurium. Proc. Natl. Acad. Sci. USA. 105 (52): 20924 - 20929 (Selected as Faculty of 1000 Biology). (2008)

Song H.W., W. Kong (Co-first author), N. Weatherspoon, G. Z. Qin, W. Tyler, J. Turk, R. Curtiss III, and Y. Shi. Modulation of the regulatory activity of bacterial two-component systems by SlyA. J. Biol. Chem., 283 (42): 28158 - 28168. (2008)

Kong, W., S.Y. Wanda, X. Zhang, W. Bollen, S.A. Tinge, K.L. Roland, and R. Curtiss III. Regulated programmed lysis of recombinantSalmonella in host tissues to release protective antigens and confer biological containment. Proc. Natl. Acad. Sci. USA. 105 (27): 9361 - 9366 (Highlighted in “In This Issue” of PNAS). (2008)

Kong, W., N. Weatherspoon, and Y. Shi. Molecular mechanism for establishment of signal - dependent regulation in the PhoP/PhoQ system. J. Biol. Chem., 283 (24): 16612 - 16621. (2008) 

Publications
  • Brenneman, K. C. Willingham, W. Kong, R. Curtiss 3rd, and K. Roland. Low pH Rescue of Acid-Sensitive Salmonella Typhi Strains by a Rhamnose-Regulated Arginine Decarboxylase System. J. Bacteriol (2013).
  • Kong, W, J. E. Clark - Curtiss, and R. Curtiss 3rd. Utilizing Salmonella for antigen delivery: the aims and benefits of bacterial delivered vaccination. Expert Review of Vaccines (2013).
  • Kong, W, J. E. Clark - Curtiss, and R. Curtiss 3rd. Utilizing Salmonella for antigen delivery: the aims and benefits of bacterial delivered vaccination. (2013).
  • Kong, W, M. Brovold, B. A. Koeneman, J. E. Clark - Curtiss, and R. Curtiss III. Turning self - destructing Salmonella into a universal DNA vaccine delivery platform. Proc. Natl. Acad. Sci. USA. 109 (47): 19414 - 19419 (2012).
  • G. Zhao, W. Kong (Co-first author), Weatherspoon-Griffin, N., J. E. Clark-Curtiss and Y. Shi. Premature transcription termination requires Mg2+-enhanced MgtL translation within Mg2+ riboswitch. EMBO Journal (2011).
  • Shamaila Ashraf; Wei Kong; Shifeng Wang; Jiseon Yang; Roy Curtiss III. Protective cellular responses elicited by vaccination with influenza nucleoprotein delivered by a live recombinant attenuated Salmonella vaccine. Vaccine (2011).
  • Weatherspoon-Griffin, N., G. Zhao, W. Kong, Y. Kong, M. Morigen, H. Andrews-Polymenis, M. McClelland, and Y. Shi. The CpxR/CpxA two-component system upregulates two Tat-dependent peptidoglycan amidases to confer bacterial resistance to antimicrobial peptide. Journal of Biological Chemistry (2011).
  • Xiangmin Zhang; Soo-Young Wanda; Karen Brenneman; Wei Kong; Xin Zhang; Kenneth Roland; Roy Curtiss III. Improving Salmonella vector with rec mutation to stabilize the DNA cargoes. BMC Microbiology (2011).
  • Benson L., and W. Kong. Investigate and characterize the means to reduce the toxicity of genetically engineered cancer therapeutic recombinant attenuated Salmonella. SOLUR: 19th Annual Undergraduate Research Poster Symposium. March 30, 2012. Life Sciences, C Wing Atrium, ASU (0).
  • Brenneman K. E., C. Willingham, J. C. Jensen, W. Kong, R. Curtiss III, and K. L. Roland. Regulated Arginine Decarboxylase System Increases Survival of Acid-Sensitive Vaccine Strains of Salmonella Typhi During Low pH Challenge. ASM 112th General Meeting, June 16-19, 2012, San Francisco, California (0).
  • Brovold M. and W. Kong. Turning Self-destructing Salmonella into Anti-cancer therapy agent. Arizona Bio-industry Association (AZBio) Annual Awards and Expo-student showcase. October 13, 2011. Sheraton Wild Horse Pass, Chandler, AZ (0).
  • Brovold M., and W. Kong. Turning Self-destructing Salmonella into Anti-cancer therapy agent. Arizona Bio-industry Association (AZBio) Annual Awards and Expo-student showcase. October 13, 2011. Sheraton Wild Horse Pass, Chandler, AZ (0).
  • Kong W., M. Brovold, J. Tully, L. Benson, and R. Curtiss III. Reprogramming Salmonella Chemotaxis System by Rhamnose-Regulating and/or Constitutive Up-regulating Synthesis of Their Chemoreceptors for Therapeutic Use. ASM 112th General Meeting, June 16-19, 2012, San Francisco, California (0).
  • Kong, W. and R. Curtiss III. Engineering a Bacterial Antigen and DNA Vaccine Delivery System. World Vaccine Congress & Expo Washington 2013 (0).
Research Activity
  • Shi,Yixin*, Kong,Wei. Study of the PorX-PorY Two-component System in Porphyromonas Gingivalis. HHS-NIH-NIDCR(3/1/2015 - 2/28/2019).
  • Curtiss III,Roy*, Kong,Wei. Attenuated Salmonella vaccines against Eimeria. (3/22/2013 - 5/31/2014).
  • Curtiss III,Roy*, Kong,Wei, Roland,Kenneth L. Recombinant Attenuated Bacterial Vaccines Against Biodefense Agents. HHS-NIH-NIAID(5/1/2011 - 4/30/2015).
  • Kong,Wei*. Engineered self-destructing Salmonella as a colorectal cancer cure. HHS-NIH-NCI(6/1/2010 - 5/31/2013).
Fall 2019
Course NumberCourse Title
BIO 492Honors Directed Study
MIC 495Undergraduate Research
BIO 495Undergraduate Research
Summer 2019
Course NumberCourse Title
MIC 495Undergraduate Research
Spring 2019
Course NumberCourse Title
BIO 493Honors Thesis
MIC 495Undergraduate Research
MBB 495Undergraduate Research
Fall 2018
Course NumberCourse Title
BIO 492Honors Directed Study
MIC 495Undergraduate Research
Summer 2018
Course NumberCourse Title
MIC 495Undergraduate Research
Spring 2018
Course NumberCourse Title
BIO 493Honors Thesis
MIC 495Undergraduate Research
MBB 495Undergraduate Research
Fall 2017
Course NumberCourse Title
BIO 492Honors Directed Study
MIC 495Undergraduate Research
Summer 2017
Course NumberCourse Title
MIC 495Undergraduate Research
Spring 2017
Course NumberCourse Title
BIO 493Honors Thesis
MIC 495Undergraduate Research
Fall 2016
Course NumberCourse Title
BIO 492Honors Directed Study
MIC 495Undergraduate Research
Summer 2016
Course NumberCourse Title
MIC 495Undergraduate Research
Spring 2016
Course NumberCourse Title
MIC 495Undergraduate Research
Fall 2015
Course NumberCourse Title
MIC 495Undergraduate Research
Summer 2015
Course NumberCourse Title
MIC 495Undergraduate Research
Spring 2015
Course NumberCourse Title
MIC 495Undergraduate Research
Service
  • Post - Research Education Program (PREP), Mentor (2012 - Present)
  • Journal Applied Microbiology and Biotechnology, Invited reviewer (2012 - Present)
  • SOLUR program, ASU, Mentor (2012 - Present)
  • The Barrett Honors College, ASU, Chair of the undergraduate honor thesis committee (2012 - Present)
  • Vaccine, Invited reviewer (2012 - Present)
  • SOLUR program, Mentor (2011 - Present)
  • Avian Disease, Invited reviewer (2011 - Present)
  • NIH, Invited member of the review panel, Indo-US Collaborative Program (NIH) (2013 - 2013)
  • SOLUR program, Mentor (2011 - 2012)