Associate Professor, Biodesign Institute;  Department of Chemistry and Biochemistry, College of Liberal Arts and Sciences

Office: BDA 124A, Lab: BDA 115
Phone: 480-727-8586, Lab Phone: 480-727-0428
Fax: 480-965-2747

Education

Ph.D., University of Buenos Aires, 1998.
Postdoctoral Researcher, UCLA, 1998-2000 and UC Berkeley 2000-2004
B.S., Chemistry, University of Buenos Aires, Argentina, 1995.

Research Areas

Physical Chemistry and Biophysics

Research and Teaching Interests

Marcia Levitus' research group focuses on the development and application of state-of-the-art techniques of single molecule detection to study complex biological systems. They use an interdisciplinary approach that interweaves concepts from physics, chemistry and biology.

Conformational dynamics of biomolecules: In contrast with the more conventional fluorescence techniques, where billions of molecules are sampled simultaneously, single molecule techniques allow the observation of subpopulations and rare events that would otherwise be hidden in the measured average. More importantly, the observation of an individual molecule allows for the study of dynamic aspects of conformational changes without the need to synchronize the entire sample. A related technique, Fluorescence Correlation Spectroscopy, is based on the analysis of the fluctuations in the fluorescent signal of a small number of molecules. Correlation analysis of the fluorescence fluctuations yields kinetic information about the dynamic processes that cause the changes in the fluorescent signal.

The research group uses these concepts to investigate the dynamics, structure and kinetics of nucleoprotein assemblies. Specific projects in this line of research include the study of the dynamic aspects of DNA-protein interactions in nucleosomes. The group is interested in characterizing the spontaneous DNA unwrapping and re-wrapping kinetics, and in the study of the effect of ATP-dependent remodeling enzymes.

Photophysical properties of fluorescent dyes commonly used in single molecule spectroscopy: They are also interested in studying the photochemical and photophysical properties of fluorescent dyes that are commonly used for single-molecule applications. Photophysical processes are a source of artifacts that have not been thoroughly explored in many cases. Photochemical reactions, such as isomerizations, produce results that can be interpreted as conformational changes of the macromolecule to which the fluorophore is attached. A careful characterization of the photophysical properties of these fluorophores is critical for correct interpretation of experimental results.

Selected Publications

1. "Demystifying PIFE: The Photophysics Behind the Protein-Induced Fluorescence Enhancement Phenomenon in Cy3".

Elana M. S. Stennett, Monika A. Ciuba, Su Lin,  Marcia Levitus.  J. Phys. Chem. Lett   2015 .  6 1819-1823

2. "Intrinsic stability and oligomerization dynamics of DNA processivity clamps".

Binder, J. K., Douma, L.G., Ranjit, S., Kanno D.M., Chakraborty, M., Bloom, L.B., and  Levitus M   Nucl. Acids. Res. 2014 .  42 , 6476-6486. 

3.   "Protein Oligomerization Monitored by Fluorescence Fluctuation Spectroscopy: Self-Assembly of Rubisco Activase".

Chakraborty,M., Kuriata, A.M., Henderson, J.N., Salvucci, M.E., Wachter, R.M.and  Levitus M   Biophys. J.   2012 .  103 , 949-958

4. "Fluorescence Properties and Photophysics of the Sulfoindocyanine Cy3 Linked Covalently to DNA".

Sanborn M.E., Connolly B.K., Gurunathan K.,  Levitus M .  J. Phys. Chem. B  2007 .  111 , 1064.