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Raimund Fromme

Research Associate Professor
Faculty, TEMPE Campus, Mailcode 1604
Faculty Member
Faculty, TEMPE Campus, Mailcode 1604

Raimuch Fromme's research has investigated the inner sanctum of photosynthesis, the structure and function of Photosystem I and II, since is master's and doctorate work. Since 2004, he has had the opportunity to work on crystal structures of various proteins in the broad field of photosynthesis, where the membrane proteins are the most interesting and challenging proteins of all. Currently the protein data bank has more than 150,000 structures, in contrast the number of known unique membrane protein structures, which hovers around 850. This means that the structure of some of the most important proteins are in their majority (one third of all proteins) still remain unknown. The field of membrane protein structure determination is still in its beginnings, with a clear growing impact on many research topics in chemistry, biochemistry, biology and medicine. Diffraction with the X-ray femtosecond Free Electron Laser(FEL) is the newest tool to provide information about the dynamic change of structures like photosystem II or the photoactive yellow protein(PYP).


Ph.D. Chemistry, Technical University of Berlin 1994

Research Interests

The inner sanctum of Photosynthesis the structure and function of Photosystem I and II is since my master thesis and PhD the focus of my research interest. Since 2004  I have the opportunity to work on crystal structures of various proteins in the broad field of photosynthesis. The membrane proteins are the most interesting and challenging proteins at all. Currently the protein data bank has above 150,000 structures, in contrast the number of known unique membrane protein structures is still around  850 . Therefore the most important proteins are in their majority still unknown by their structure, beside the fact that the numeric share is around one third of all proteins. The field of membrane protein structure determination is  still in the beginning with a clear growing impact to many research topics in chemistry, biochemistry, biology and medicine. Diffraction with the X-ray femtosecond Free Electron Laser(FEL) is the newest tool to provide information about the dynamic change of structures like photosystem II or the photoactive yellow protein(PYP).

Article I have been corresponding author :

Gisriel et al. (2017) Science accepted July 28 online

Articles I contributed as co author:

Chapman et al. (2011)  Nature  470,    73–77.

Boutet et al.(2012) Science  337(6092):362-4.

Redecke et al. (2013) Science 339(6116):227-30

Liu et al. (2013) Science 20 December 2013: 1521-1524

Kupitz et al. (2014) Nature 9 July doi:10.1038/nature13453

Tenboer et al. (2014) Science 5 December : 1242-1246. [DOI:10.1126/science.1259357]

Kang et al. (2015) Nature doi:10.1038/nature14656, online 22 July 2015

Ayyer et al. (2016) Nature, 530, 202-206 Macromolecular diffractive imaging using imperfect crystals

Pande et al.(2016) Science 352, 725-729,  DOI: 10.1126/science.aad5081



Articles in mostly peer-reviewed Journals

58. Snapshot of an oxygen intermediate in the catalytic reaction of cytochrome c oxidase

Izumi Ishigami, Ariel Lewis-Ballester, Austin Echelmeier, Gerrit Brehm, Nadia A. Zatsepin, Thomas D. Grant, Jesse D. Coe, Stella Lisova, Garrett Nelson, Shangji Zhang, Zachary F. Dobson, Sébastien Boutet, Raymond G. Sierra, Alexander Batyuk, Petra Fromme, Raimund Fromme, John C. H. Spence, Alexandra Ros, Syun-Ru Yeh, Denis L. Rousseau

Proceedings of the National Academy of Sciences Feb 2019, 201814526; DOI: 10.1073/pnas.1814526116

57. X-ray Emission Spectroscopy at X-ray Free Electron Lasers: Limits to Observation of the Classical Spectroscopic Response for Electronic Structure Analysis

Scott Jensen, Brendan T Sullivan, Daniel A Hartzler, Jose Meza Aguilar, Salah Awel, Sasa Bajt, Shibom Basu, Richard Bean, Henry Chapman, Chelsie Conrad, Matthias Frank,Raimund Fromme , Jose M Martin-Garcia, Thomas D Grant, Michael Heymann, Mark S Hunter, Gihan Ketawala, Richard Adam Kirian, Juraj Knoska, Christopher Kupitz, Xuanxuan Li, Mengning Liang, Stella Lisova, Valerio Mariani, Victoria Leonidovna Mazalova, Marc Messerschmidt, Michael Moran, Garrett Nelson, Dominik Oberthuer, Alex Schaffer, Raymond G Sierra, Natalie Vaughn, Uwe Weierstall, Max O Wiedorn, P Lourdu Xavier, Jay-How Yang, Oleksandr Yefanov, Nadia Zatsepin, Andrew L Aquila, Petra Fromme, Sebastien Boutet, Gerald T Seidler, Yulia N Pushkar


The Journal of Physical Chemistry Letters DOI: 10.1021/acs.jpclett.8b03595 (Web): December 19, 2018

56. Free-electron laser data for multiple-particle fluctuation scattering analysis

Kanupriya Pande, Jeffrey J Donatelli, Erik Malmerberg, Lutz Foucar, Billy K Poon, Markus Sutter, Sabine Botha, Shibom Basu, R Bruce Doak, Katerina Dörner, Sascha W Epp, Lars Englert, Raimund Fromme, Elisabeth Hartmann, Robert Hartmann, Guenter Hauser, Johan Hattne, Ahmad Hosseinizadeh, Stephan Kassemeyer, Lukas Lomb, Sebastian F Carron Montero, Andreas Menzel, Daniel Rolles, Artem Rudenko, Marvin M Seibert, Raymond George Sierra, Peter Schwander, Abbas Ourmazd, Petra Fromme, Nicholas K Sauter, Michael Bogan, John Bozek, Christoph Bostedt, Ilme Schlichting, Cheryl A Kerfeld, Petrus H Zwart


Scientific Data volume 5, Article number: 180201 (2018)

55. Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography

Jose L Olmos, Suraj Pandey, Jose M Martin-Garcia, George Calvey, Andrea Katz, Juraj Knoska, Christopher Kupitz, Mark S Hunter, Mengning Liang, Dominik Oberthuer, Oleksandr Yefanov, Max Wiedorn, Michael Heyman, Mark Holl, Kanupriya Pande, Anton Barty, Mitchell D Miller, Stephan Stern, Shatabdi Roy-Chowdhury, Jesse Coe, Nirupa Nagaratnam, James Zook, Jacob Verburgt, Tyler Norwood, Ishwor Poudyal, David Xu, Jason Koglin, Matthew H Seaberg, Yun Zhao, Saša Bajt, Thomas Grant, Valerio Mariani, Garrett Nelson, Ganesh Subramanian, Euiyoung Bae, Raimund Fromme, Russell Fung, Peter Schwander, Matthias Frank, Thomas A White, Uwe Weierstall, Nadia Zatsepin, John Spence, Petra Fromme, Henry N Chapman, Lois Pollack, Lee Tremblay, Abbas Ourmazd, George N Phillips, Marius Schmidt


BMC biology (2018)16 (1), 59



Christopher Gisriel, Iosifna Sarrou, Bryan Ferlez, John H. Golbeck, Kevin E. Redding, Raimund Fromme

Science2017 ahead of printed release July 27, doi:10.1126/science.aan5611

53. Crystal structure of CO-bound cytochrome c oxidase determined by serial femtosecond X-ray crystallography at room temperature

Izumi Ishigami, Nadia A. Zatsepin, Masahide Hikita, Chelsie E. Conrad, Garrett Nelson, Jesse D. Coe, Shibom Basu, Thomas D. Grant, Matthew H. Seaberg, Raymond G. Sierra, Mark S. Hunter, Petra Fromme, Raimund Fromme, Syun-Ru Yeh, and Denis L. Rousseau

PNAS2017 ; published ahead of print July 11, 2017, doi:10.1073/pnas.1705628114

52. Diffraction data of core-shell nanoparticles from an X-ray free electron laser

Xuanxuan Li, Chun-Ya Chiu, Hsiang-Ju Wang, Stephan Kassemeyer, Sabine Botha, Robert L. Shoeman, Robert M. Lawrence, Christopher Kupitz, Richard Kirian, Daniel James, Dingjie Wang, Garrett Nelson, Marc Messerschmidt, Sébastien Boutet, Garth J. Williams, Elisabeth Hartmann, Aliakbar Jafarpour, Lutz M. Foucar, Anton Barty, Henry Chapman, Mengning Liang, Andreas Menzel, Fenglin Wang, Shibom Basu, Raimund Fromme, R. Bruce Doak, Petra Fromme, Uwe Weierstall, Michael H. Huang, John C. H. Spence, Ilme Schlichting, Brenda G. Hogue and Haiguang Liu

Sci Data 2017; 4: 170048.Published online 2017 Apr 11. doi: 10.1038/sdata.2017.48

51. Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser

Cornelius Gati, Dominik Oberthuer, Oleksandr Yefanov, Richard D. Bunker, Francesco Stellato, Elaine Chiu, Shin-Mei Yeh, Andrew Aquila, Shibom Basu, Richard Bean, Kenneth R. Beyerlein, Sabine Botha, Sébastien Boutet, Daniel P. DePonte, R. Bruce Doak, Raimund Fromme, Lorenzo Galli, Ingo Grotjohann, Daniel R. James, Christopher Kupitz, Lukas Lomb, Marc Messerschmidt, Karol Nass, Kimberly Rendek, Robert L. Shoeman, Dingjie Wang, Uwe Weierstall, Thomas A. White, Garth J. Williams, Nadia A. Zatsepin, Petra Fromme, John C. H. Spence, Kenneth N. Goldie, Johannes A. Jehle, Peter Metcalf, Anton Barty, and Henry N. Chapman,


PNAS Feb 15, 2017

50. Structural enzymology using X-ray free electron lasers

Christopher Kupitz, Jose L Olmos Jr, Mark Holl, Lee Tremblay, Kanupriya Pande, Suraj Pandey, Dominik Oberthür, Mark Hunter, Mengning Liang, Andrew Aquila, Jason Tenboer, George Calvey, Andrea Katz, Yujie Chen, Max O Wiedorn, Juraj Knoska, Alke Meents, Valerio Majriani, Tyler Norwood, Ishwor Poudyal, Thomas Grant, Mitchell D Miller, Weijun Xu, Aleksandra Tolstikova, Andrew Morgan, Markus Metz, Jose M Martin-Garcia, James D Zook, Shatabdi Roy-Chowdhury, Jesse Coe, Nirupa Nagaratnam, Domingo Meza, Raimund Fromme, Shibom Basu, Matthias Frank, Thomas White, Anton Barty, Sasa Bajt, Oleksandr Yefanov, Henry N Chapman, Nadia Zatsepin, Garrett Nelson, Uwe Weierstall, John Spence, Peter Schwander, Lois Pollack, Petra Fromme, Abbas Ourmazd, George N Phillips Jr, Marius Schmidt

49. Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

Pande, K., Hutchison, C. D. M., Groenhof, G., Aquila, A., Robinson, J. S., Tenboer, J., Basu, S., Boutet, S., DePonte, D. P., Liang, M., White, T. A., Zatsepin, N. A., Yefanov, O., Morozov, D., Oberthuer, D., Gati, C., Subramanian, G., James, D., Zhao, Y., Koralek, J., Brayshaw, J., Kupitz, C., Conrad, C., Roy-Chowdhury, S., Coe, J. D., Metz, M., Xavier, P. L., Grant, T. D., Koglin, J. E., Ketawala, G., Fromme, R., Srajer, V., Henning, R., Spence, J. C. H., Ourmazd, A., Schwander, P., Weierstall, U., Frank, M., Fromme, P., Barty, A., Chapman, H. N., Moffat, K., van Thor, J. J., and Schmidt, M.

Science 2016 May 6;352(6286):725-9. doi: 10.1126/science.aad5081. Epub 2016 May 5.

48. Protein Crystallization in an Actuated Microfluidic Nanowell Device

Bahige G. Abdallah, Shatabdi Roy-Chowdhury, Raimund Fromme, Petra Fromme, and Alexandra Ros

Crystal Growth & Design 2016, 16 (4), pp 2074–2082 DOI: 10.1021/acs.cgd.5b01748 Publication Date (Web): February 25, 2016

47. Macromolecular diffractive imaging using imperfect crystals

Kartik Ayyer,Oleksandr M. Yefanov,Dominik Oberthür, Shatabdi Roy-Chowdhury, Lorenzo Galli, Valerio Mariani, Shibom Basu, Jesse Coe, Chelsie E. Conrad, Raimund Fromme, Alexander Schaffer, Katerina Dörner, Daniel James, Christopher Kupitz, Markus Metz, Garrett Nelson, Paulraj Lourdu Xavier, Kenneth R. Beyerlein, Marius Schmidt, Iosifina Sarrou, John C. H. Spence, Uwe Weierstall,Thomas A. White,Jay-How Yang,Yun Zhao,Mengning Liang,Andrew Aquila, Mark S. Hunter, Joseph S. Robinson, Jason E. Koglin, Sébastien Boutet, Petra Fromme, Anton Barty& Henry N. Chapman

Nature 530, 202–206 (11 February 2016) doi:10.1038/nature16949

46. Ternary structure reveals mechanism of a membrane diacylglycerol kinase

Dianfan Li, Phillip J. Stansfeld, Mark S.P. Sansom, Aaron Keogh, Lutz Vogeley, Nicole Howe, Joseph A. Lyons, David Aragao, Petra Fromme, Raimund Fromme, Shibom Basu, Ingo Grotjohann, Christopher Kupitz, Kimberley Rendek, Uwe Weierstall, Nadia A. Zatsepin, Vadim Cherezov, Wei Liu, Sateesh Bandaru, Niall J. English, Cornelius Gati, Anton Barty, Oleksandr Yefanov, Henry N. Chapman, Kay Diederichs, Marc Messerschmidt, Sebastien Boutet, Garth J. Williams, M. Marvin Seibert & Martin Caffrey NATURE COMMUNICATIONS (2015)|6:10140|DOI: 10.1038/ncomms10140| Published 17 Dec 2015

45. Serial femtosecond crystallography of soluble proteins in lipidic cubic phase

Raimund Fromme, Andrii Ishchenko,Markus Metz, Shatabdi Roy Chowdhury, Shibom Basu, Sébastien Boutet, Petra Fromme, Thomas A. White, Anton Barty, John C. H. Spence, Uwe Weierstall, Wei Liu and Vadim Cherezov

IUCrJ, Volume 2, 5, September 2015, doi:10.1107/S2052252515013160

44. Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

Kang Y, Zhou XE, Gao X, He Y, Liu W, Ishchenko A, Barty A, White TA, Yefanov O, Han GW, Xu Q, de Waal PW, Ke J, Tan MH, Zhang C, Moeller A, West GM, Pascal BD, Van Eps N, Caro LN, Vishnivetskiy SA, Lee RJ, Suino-Powell KM, Gu X, Pal K, Ma J, Zhi X, Boutet S, Williams GJ, Messerschmidt M, Gati C, Zatsepin NA, Wang D, James D, Basu S, Roy-Chowdhury S, Conrad CE, Coe J, Liu H, Lisova S, Kupitz C, Grotjohann I, Fromme R, Jiang Y, Tan M, Yang H, Li J, Wang M, Zheng Z, Li D, Howe N, Zhao Y, Standfuss J, Diederichs K, Dong Y, Potter CS, Carragher B, Caffrey M, Jiang H, Chapman HN, Spence JC, Fromme P, Weierstall U, Ernst OP, Katritch V, Gurevich VV, Griffin PR, Hubbell WL, Stevens RC, Cherezov V, Melcher K, Xu HE

Nature (2015) doi:10.1038/nature14656, online 22 July 2015

43. Chapter Twenty-Two – Crystallization of Photosystem II for Time-Resolved Structural Studies Using an X-ray Free Electron Laser

Jesse Coe, Christopher Kupitz, Shibom Basu, Chelsie E. Conrad, Shatabdi Roy-Chowdhury, Raimund Fromme, Petra Fromme

Methods Enzymol. 2015;557:459-82. doi: 10.1016/bs.mie.2015.01.011. Epub 2015 Apr 18.

42. A novel inert crystal delivery medium for serial femtosecond crystallography

Chelsie E. Conrad, Shibom Basu, Daniel James, Dingjie Wang, Alexander Schaffer, Shatabdi Roy-Chowdhury, Nadia A. Zatsepin, Andrew Aquila, Jesse Coe, Cornelius Gati, Mark S. Hunter, Jason E. Koglin, Christopher Kupitz, Garrett Nelson, Ganesh Subramanian, Thomas A. White, Yun Zhao, James Zook, Sébastien Boutet, Vadim Cherezov, John C. H. Spence, Raimund Fromme, Uwe Weierstall, and Petra Fromme

IUCrJ Volume 2| Part 4| July 2015| Pages 421-430 ISSN: 2052-2525, doi:10.1107/S2052252515009811

41. Structural basis for bifunctional peptide recognition at human δ-opioid receptor

Gustavo Fenalti,Nadia A Zatsepin,Cecilia Betti,Patrick Giguere,Gye Won Han,Andrii Ishchenko,Wei Liu,Karel Guillemyn,Haitao Zhang,Daniel James,Dingjie Wang,Uwe Weierstall,John C H Spence,Sébastien Boutet,Marc Messerschmidt,Garth J Williams,Cornelius Gati,Oleksandr M Yefanov,Thomas A White,Dominik Oberthuer,Markus Metz,Chun Hong Yoon,Anton Barty,Henry N Chapman, Shibom Basu,Jesse Coe,Chelsie E Conrad,Raimund Fromme,Petra Fromme,Dirk Tourwé,Peter W Schiller, Bryan L Roth,Steven Ballet,Vsevolod Katritch,Raymond C Stevens & Vadim Cherezov

Nature Structural & Molecular Biology (2015) doi:10.1038/nsmb.2965

40. A Hinge Migration Mechanism Unlocks the Evolution of Green-to-Red Photoconversion in GFP-like Proteins

Hanseong Kim, Taisong Zou, Chintan Modi, Katerina Dörner, Timothy J. Grunkemeyer, Liqing Chen, Raimund Fromme, Mikhail V. Matz, S. Banu Ozkan and Rebekka M. Wachter

Structure 2015, Vol 23,1,34-43 doi:10.1016/j.str.2014.11.011

39. Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

Jason Tenboer, Shibom Basu, Nadia Zatsepin, Kanupriya Pande, Despina Milathianaki, Matthias Frank, Mark Hunter, Sébastien Boutet, Garth J. Williams, Jason E. Koglin, Dominik Oberthuer, Michael Heymann, Christopher Kupitz, Chelsie Conrad, Jesse Coe,Shatabdi Roy-Chowdhury, Uwe Weierstall, Daniel James, Dingjie Wang, Thomas Grant,Anton Barty, Oleksandr Yefanov,Jennifer Scales, Cornelius Gati, Carolin Seuring, Vukica Srajer, Robert Henning, Peter Schwander, Raimund Fromme, Abbas Ourmazd, Keith Moffat, Jasper J. Van Thor, John C. H. Spence, Petra Fromme, Henry N. Chapman and Marius Schmidt

Science December 2014: 1242-1246. [DOI:10.1126/science.1259357]

38. Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1

Ho-Hsien Lee, Irene Cherni, HongQi Yu, Raimund Fromme, Jeffrey D. Doran, Ingo Grotjohann, Michele Mittman, Shibom Basu, Arpan Deb, Katerina Dörner, Andrew Aquila, Anton Barty, Sébastien Boutet, Henry N. Chapman, R. Bruce Doak, Mark S. Hunter, Daniel James, Richard A. Kirian, Christopher Kupitz, Robert M. Lawrence, Haiguang Liu, Karol Nass, Ilme Schlichting, Kevin E. Schmidt, M. Marvin Seibert, Robert L. Shoeman, John C. H. Spence, Francesco Stellato, Uwe Weierstall, Garth J. Williams, Chunhong Yoon, Dingjie Wang, Nadia A. Zatsepin, Brenda G. Hogue, Nobuyuki Matoba, Petra Fromme and Tsafrir S. Mor

IUCrJSep 1, 2014; 1(Pt 5): 305–317. Published online Aug 20, 2014. doi: 10.1107/S2052252514014900


37. Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser

David Arnlund, Linda C Johansson, Cecilia Wickstrand, Anton Barty, Garth J Williams, Erik Malmerberg, Jan Davidsson, Despina Milathianaki, Daniel P DePonte, Robert L Shoeman, Dingjie Wang, Daniel James, Gergely Katona, Sebastian Westenhoff, Thomas A White, Andrew Aquila,Sadia Bari, Peter Berntsen, Mike Bogan, Tim Brandt vanDriel,R Bruce Doak,Kasper SkovKjær, Matthias Frank, Raimund Fromme, Ingo Grotjohann, Robert Henning, Mark S Hunter, Richard A Kirian, Irina Kosheleva, Christopher Kupitz, Mengning Liang, Andrew V Martin, Martin Meedom Nielsen, Marc Messerschmidt, M Marvin Seibert, Jennie Sjöhamn, Francesco Stellato, Uwe Weierstall, Nadia A Zatsepin, John CH Spence, Petra Fromme, Ilme Schlichting, Sébastien Boutet, Gerrit Groenhof, Henry N Chapman & Richard Neutze

Nature Methods2014 Aug 10. doi: 10.1038/nmeth.3067. [Epub ahead of print]


36. Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser

Christopher Kupitz, Shibom Basu, Ingo Grotjohann, Raimund Fromme, Nadia A. Zatsepin, Kimberly N. Rendek, Mark S. Hunter, Robert L. Shoeman, Thomas A. White, Dingjie Wang, Daniel James, Jay-How Yang,Danielle E. Cobb,Brenda Reeder, Raymond G. Sierra, Haiguang Liu, Anton Barty, Andrew L. Aquila, Daniel Deponte, Richard A. Kirian,Sadia Bari, Jesse J. Bergkamp,Kenneth R. Beyerlein, Michael J. Bogan, Carl Caleman, Tzu-Chiao Chao,Chelsie E. Conrad, Katherine M. Davis, Holger Fleckenstein, Lorenzo Galli, Stefan P. Hau-Riege, Stephan Kassemeyer,Hartawan Laksmono, Mengning Liang, Lukas Lomb, Stefano Marchesini, Andrew V. Martin, Marc Messerschmidt Despina Milathianaki, Karol Nass, Alexandra Ros, Shatabdi Roy-Chowdhury, Kevin Schmidt, Marvin Seibert, Jan Steinbrener, Francesco Stellato, Lifen Yan, Chunhong Yoon, Thomas A. Moore, Ana L. Moore, Yulia Pushkar,Garth J. Williams, Sébastien Boutet, R. Bruce Doak, Uwe Weierstall, Matthias Frank, Henry N. Chapman,John C. H. Spence & Petra Fromme

Nature 2014 Jul 9 doi:10.1038/nature13453

35. Microcrystallization techniques for serial femtosecond crystallography using photosystem II from Thermosynechococcus elongatus as a model system

Kupitz C, Grotjohann I, Conrad CE, Roy-Chowdhury S, Fromme R, Fromme P

Philos Trans R Soc Lond B Biol Sci. 2014 Jul 17;369(1647). pii: 20130316. doi: 10.1098/rstb.2013.0316.

34. Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Uwe Weierstall, Daniel James,Chong Wang,Thomas A. White, Dingjie Wang,Wei Liu,John C. H. Spence, R. Bruce Doak, Garrett Nelson, Petra Fromme, Raimund Fromme, Ingo Grotjohann, Christopher Kupitz, Nadia A. Zatsepin, Haiguang Liu,Shibom Basu,Daniel Wacker, Gye Won Han, Vsevolod Katritch, Sébastien Boutet, Marc Messerschmidt, Garth J. Williams, Jason E. Koglin,M. Marvin Seibert, Markus Klinker, Cornelius Gati,Robert L. Shoeman, Anton Barty,Henry N. Chapman,Richard A. Kirian, Kenneth R. Beyerlein, Raymond C. Stevens, Dianfan Li, Syed T. A. Shah, Nicole Howe, Martin Caffrey & Vadim Cherezov

Nat Commun. 2014 Feb 14;5:3309. doi: 10.1038/ncomms4309.

33. X-ray crystal structure of the passenger domain of plasmid encoded toxin(Pet), an autotransporter enterotoxin from enteroaggregative Escherichia coli (EAEC)

Domingo Meza-Aguilar J, Fromme P, Torres-Larios A, Mendoza-Hernández G, Hernandez-Chiñas U, Arreguin-Espinosa de Los Monteros RA, Eslava Campos CA, Fromme R.

Biochem Biophys Res Commun. 2014 Feb 12. pii: S0006-291X(14)00268-X. doi: 10.1016/j.bbrc.2014.02.016. [Epub ahead of print]

32. Serial Femtosecond Crystallography of G Protein–Coupled Receptors

Wei Liu, Daniel Wacker, Cornelius Gati, Gye Won Han, Daniel James, Dingjie Wang, Garrett Nelson, Uwe Weierstall, Vsevolod Katritch, Anton Barty, Nadia A. Zatsepin, Dianfan Li, Marc Messerschmidt, Sébastien Boutet, Garth J. Williams, Jason E. Koglin, M. Marvin Seibert, Chong Wang, Syed T. A. Shah, Shibom Basu, Raimund Fromme, Christopher Kupitz, Kimberley N. Rendek, Ingo Grotjohann, Petra Fromme, Richard A. Kirian, Kenneth R. Beyerlein, Thomas A. White, Henry N. Chapman, Martin Caffrey, John C. H. Spence, Raymond C. Stevens, and Vadim Cherezov

 Science 20 December 2013: 1521-1524. [DOI:10.1126/science.1244142]

31. Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Linda C. Johansson, David Arnlund, Gergely Katona, Thomas A. White, Anton Barty, Daniel P. DePonte,Robert L. Shoeman, Cecilia Wickstrand, Amit Sharma, Garth J. Williams, Andrew Aquila, Michael J. Bogan, Carl Caleman, Jan Davidsson, R. Bruce Doak, Matthias Frank, Raimund Fromme, Lorenzo Galli, Ingo Grotjohann, Mark S. Hunter, Stephan Kassemeyer, Richard A. Kirian,Christopher Kupitz, Mengning Liang, Lukas Lomb, Erik Malmerberg, Andrew V. Martin, Marc Messerschmidt, Karol Nass, Lars Redecke, M. Marvin Seibert, Jennie Sjöhamn, Jan Steinbrener, Francesco Stellato, Dingjie Wang, Weixaio Y. Wahlgren, Uwe Weierstall, Sebastian Westenhoff, Nadia A. Zatsepin, Sébastien Boutet, John C.H. Spence, Ilme Schlichting, Henry N. Chapman, Petra Fromme & Richard Neutze

Nature Communications 2013,4,Article number: 2911 doi:10.1038/ncomms3911

30. Acid–Base Catalysis and Crystal Structures of a Least Evolved Ancestral GFP-like Protein Undergoing Green-to-Red Photoconversion

Hanseong Kim, Timothy J Grunkemeyer, Chintan Modi, Liqing Chen, Raimund Fromme, Mikhail V Matz, Rebekka Maria Wachter.

Biochemistry, 2013, 52 (45), pp 8048–8059 DOI: 10.1021/bi401000e

29. The 1.6 Å resolution structure of a FRET-optimized Cerulean fluorescent protein.

Watkins JL, Kim H, Markwardt ML, Chen L, Fromme R, Rizzo MA, Wachter RM.

Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):767-73. doi: 10.1107/S0907444913001546. Epub 2013 Apr 11.

28. Crystallization of a self-assembled three-dimensional DNA nanostructure

Kimberly N. Rendek, Raimund Fromme, Ingo Grotjohann and Petra Fromme

Acta Cryst.(2013). F69, 141-146 [ doi:10.1107/S1744309112052128 ]

27. Natively Inhibited Trypanosoma brucei Cathepsin B Structure Determined by Using an X-ray Laser


Lars Redecke, Karol Nass, Daniel P. DePonte, Thomas A. White, Dirk Rehders, Anton Barty, Francesco Stellato, Mengning Liang, Thomas R.M. Barends, Sébastien Boutet, Garth J. Williams, Marc Messerschmidt, M. Marvin Seibert, Andrew Aquila, David Arnlund, Sasa Bajt, Torsten Barth, Michael J. Bogan, Carl Caleman, Tzu-Chiao Chao, R. Bruce Doak, Holger Fleckenstein, Matthias Frank, Raimund Fromme, Lorenzo Galli, Ingo Grotjohann, Mark S. Hunter, Linda C. Johansson, Stephan Kassemeyer, Gergely Katona, Richard A. Kirian, Rudolf Koopmann, Chris Kupitz, Lukas Lomb, Andrew V. Martin, Stefan Mogk, Richard Neutze, Robert L. Shoeman, Jan Steinbrener, Nicusor Timneanu, Dingjie Wang, Uwe Weierstall, Nadia A. Zatsepin, John C.H. Spence, Petra Fromme, Ilme Schlichting, Michael Duszenko, Christian Betzel, and Henry N. Chapman


SciencePublished online 29 November 2012 [DOI:10.1126/science.1229663]

26. Improving the Accuracy of Macromolecular Structure Refinement at 7 Å Resolution

Axel T. Brunger, Paul D. Adams, Petra Fromme, Raimund Fromme, Michael Levitt, Gunnar F. Schröder

Structure Volume 20, Issue 6, 6 June 2012, Pages 957–966,[doi:10.1016/j.str.2012.04.020]

25. High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography.

Boutet S, Lomb L, Williams GJ, Barends TR, Aquila A, Doak RB, Weierstall U, Deponte DP, Steinbrener J, Shoeman RL, Messerschmidt M, Barty A, White TA, Kassemeyer S, Kirian RA, Seibert MM, Montanez PA, Kenney C, Herbst R, Hart P, Pines J, Haller G, Gruner SM, Philipp HT, Tate MW, Hromalik M, Koerner LJ, van Bakel N, Morse J, Ghonsalves W, Arnlund D, Bogan MJ, Caleman C, Fromme R, Hampton CY, Hunter MS, Johansson L, Katona G, Kupitz C, Liang M, Martin AV, Nass K, Redecke L, Stellato F, Timneanu N, Wang D, Zatsepin NA, Schafer D, Defever J, Neutze R, Fromme P, Spence JC, Chapman HN, Schlichting I.

Science (2012)Jul 20;337(6092):362-4. [doi: 10.1126/science.1217737]

24. Time-resolved protein nanocrystallography using an X-ray free-electron laser.

Aquila A, Hunter MS, Doak RB, Kirian RA, Fromme P, White TA, Andreasson J, Arnlund D, Bajt S, Barends TR, Barthelmess M, Bogan MJ, Bostedt C, Bottin H, Bozek JD, Caleman C, Coppola N, Davidsson J, DePonte DP, Elser V, Epp SW, Erk B, Fleckenstein H, Foucar L, Frank M, Fromme R, Graafsma H, Grotjohann I, Gumprecht L, Hajdu J, Hampton CY, Hartmann A, Hartmann R, Hau-Riege S, Hauser G, Hirsemann H, Holl P, Holton JM, Hömke A, Johansson L, Kimmel N, Kassemeyer S, Krasniqi F, Kühnel KU, Liang M, Lomb L, Malmerberg E, Marchesini S, Martin AV, Maia FR, Messerschmidt M, Nass K, Reich C, Neutze R, Rolles D, Rudek B, Rudenko A, Schlichting I, Schmidt C, Schmidt KE, Schulz J, Seibert MM, Shoeman RL, Sierra R, Soltau H, Starodub D, Stellato F, Stern S, Strüder L, Timneanu N, Ullrich J, Wang X, Williams GJ, Weidenspointner G, Weierstall U, Wunderer C, Barty A, Spence JC, Chapman HN.

Opt Express. 2012 Jan 30;20(3):2706-16. doi: 10.1364/OE.20.002706.

23. Atomic Resolution X-ray Structure of the Substrate Recognition Domain of Higher Plant Ribulose-bisphosphate Carboxylase/Oxygenase (Rubisco) Activase"

Henderson, J.N., Kuriata, A.M., Fromme, R., Salvucci, M.E. & Wachter, R.M.

Journal of Biological Chemistry, 2011 vol. 286, no. 41, pp. 35683-35688. [doi: 10.1074/jbc.C111.289595]  


22. Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals

Kirian RA, White TA, Holton JM, Chapman HN, Fromme P, Barty A, Lomb L, Aquila A, Maia FR, Martin AV, Fromme R, Wang X, Hunter MS, Schmidt KE, Spence JC

Acta Crystallogr A . 2011 Mar;67(Pt 2):131-40. Epub 2011 Feb 16,[ doi: 10.1107/S0108767310050981]



21. Femtosecond X-Ray Protein Nanocrystallography.

Chapman, H. N., Fromme, P., Barty, A., White, T. A., Kirian, R. A., Aquila, A., Hunter, M. S., Schulz, J., DePonte, D. P., Weierstall, U., Doak, R. B., Maia, F. R. N. C., Martin, A. V., Schlichting, I., Lomb, L., Coppola, N., Shoeman, R. L., Epp, S. W., Hartmann, R., Rolles, D., Rudenko, A., Foucar, L., Kimmel, N., Weidenspointner, G., Holl, P., Liang, M., Barthelmess, M., Caleman, C., Boutet, S., Bogan, M. J., Krzywinski, J., Bostedt, C., Bajt, S., Gumprecht, L., Rudek, B., Erk, B., Schmidt, C., Hoemke, A., Reich, C., Pietschner, D., Strueder, L., Hauser, G., Gorke, H., Ullrich, J., Herrmann, S., Schaller, G., Schopper, F., Soltau, H., Kuehnel, K., Messerschmidt, M., Bozek, J. D., Hau-Riege, S. P., Frank, M., Hampton, C. Y., Sierra, R. G., Starodub, D., Williams, G. J., Hajdu, J., Timneanu, N., Seibert, M. M., Andreasson, J., Rocker, A., Joensson, O., Svenda, M., Stern, S., Nass, K., Andritschke, R., Schroeter, C., Krasniqi, F., Bott, M., Schmidt, K. E., Wang, X., Grotjohann, I., Holton, J. M., Barends, T. R. M., Neutze, R., Marchesini, S., Fromme, R., Schorb, S., Rupp, D., Adolph, M., Gorkhover, T., Andersson, I., Hirsemann, H., Potdevin, G., Graafsma, H., Nilsson, B., and Spence, J. C. H.

Nature (2011) 470 , 73-77,[doi: 10.1038/nature09750]


20. Purification, characterization and crystallization of menaquinol:fumarate oxidoreductase from the green filamentous photosynthetic bacterium Chloroflexus aurantiacus

Yueyong Xin, Yih-Kuang Lu, Raimund Fromme, Petra Fromme and Robert E. Blankenship

Biochimica et Biophysica Acta(BBA) - Bioenergetics(2009),Volume 1787, pp. 86-96,[doi:10.1016/j.bbabio.2008.11.010]


19. Photosynthetic protein complexes, Editor: P.Fromme, Ferredoxin and Flavodoxin mediated Electron Transfer in Photosystem I. Raimund Fromme(2008)in:  Bookchapter. Wiley-VCH, Weinheim, pp. 341-352[DOI: 10.1002/9783527623464.ch15]

18. Crystallization of the c14-rotor of the chloroplast ATP synthase reveals that it contains pigments

Benjamin Varco-Merth, Raimund Fromme, Meitian Wang and Petra Fromme 

Biochimica et Biophysica Acta (BBA) - Bioenergetics,(2008) Volume 1777, Issues 7-8, Pages 605-612[doi:10.1016/j.bbabio.2008.05.009]


17. Conformational gating of sugar binding to the antiviral protein cyanovirin revealed from the crystal structure at 1.35 Å resolution

Raimund Fromme, Zivile Katiliene, Petra Fromme, Giovanna Ghirlanda

Protein Science 17: 939-944[DOI: 10.1110/ps.083472808]


16. A Monovalent Mutant of Cyanovirin-N Provides Insight Into the Role of Multiple Interactions with gp120 for Antiviral Activity

Fromme, R. Katilene,Z. Giomarelli,B. Bogani, F. McMahon, J. Mori, T. , Fromme, P. Ghirlanda, G

BIOCHEMISTRY (2007)46, 9199-9207,DOI: 10.1021/bi700666m

15. Microsecond Time Scale Rotation Measurements of Single F1-ATPase Molecules

Spetzler, D., York, J., Daniel, D. ,Fromme, R., Lowry, D. & Frasch ,W

BIOCHEMISTRY (2006) 45 (10): 3117-3124[DOI: 10.1021/bi052363n]


14. Fromme R, Yu H, Grotjohann I, Fromme P.(2005)Cocrystals of photosystem I with its soluble natural electron acceptor ferredoxin at 4 A resolution. FEBS JOURNAL 272, 449


13. Fromme P, Yu H, Bukman Y, Ni D, Varco-Merth B, Chauhan D, Vanselow C, Jolley C, Fromme R, Grotjohann I(2005) New Insigths into the structure and function of photosystem I and II. FEBS JOURNAL 272, 451

12. Structure and Function of Photosystem I

Fromme, R., Grotjohann, I.,&Fromme, P (2007)Structure and function of Photosystem I. Editor G. Renger . Royal Society of Chemistry   (Cambridge, UK)    111-146,DOI:10.1039/9781847558169-00111


11. Fromme, R., Grotjohann, I.,&Fromme, P (2006) Primary Processes in Photosynthesis. Editor R. Grisshammer and S. Buchanan.  Royal Society of Chemistry (Cambridge, UK)  ISBN 0 85404 361 6, Bookchapter. pp. 320-348 Structure of Photosystems I and II 


10. Fromme, Raimund(1994)  Untersuchungen  zur Struktur und Funktion des Photosystems  II der Photosynthese aus Höheren Pflanzen und Algen, Verlag Dr. Köster Berlin, ISBN 3-89574-030-6, 159 pages

9. Fromme, R., Hohm-Veit, S., & Renger, G. (1992). Stabilization of Photosystem II with BSA and Immobilization with Alginate. Research in Photosynthesis (Murata, N. ed.)Vol. 2, pp.183-186 Kluwer, Dordrecht

8. Fromme, R., & Renger, G. (1990). Studies on Herbicide Binding in Photosystem II Membrane Fragments from Spinach. Z. Naturforsch., 45 c, 373-378

7. Gleiter, H. M., Ohad, N., Hirschberg, J.,Fromme, R., Renger, G., Koike, H., & Inoue, Y. (1990). An Application of Thermoluminescence to Herbicide Studies.  Z. Naturforsch., 45 c, 353-358

6. Schmid, R., Fromme, R., & Renger, G. (1990). The photosynthetic apparatus of Acetabularia mediterranea grown under red and blue light.  Biophysical quantification and characterization of  Photosystem II and its core components.  Photochem. Photobiol., 52, 103-109

5. Renger, G., Messinger, J., & Fromme, R. (1989). Tribromotoluquinone Induced Modification of the Oscillation Pattern of Oxygen Evolution and of Herbicide Binding in Thylakoids and PS 2 Membrane Fragments from Spinach. Z. Naturforsch., 44 c, 423-430

4. Renger, G., Völker, M., Eckert, H.-J., Fromme, R., Hohm-Veit, S., & Gräber, P. (1989). On the mechanism of Photosystem II deterioration by UV-B irradiation. Photochem. Photobiol., 49(1), 97-105

3. Renger, G., Fromme, R., & Hagemann, R. (1988). The modification of atrazine binding by the redox state of the endogenous high-spin iron and by specific proteolytic enzymes in Photosystem membrane fragments and intact thylakoids. Biochim. Biophys. Acta, 935, 173-183

2. Fromme, R., Hagemann, R., & Renger, G. (1987). Comparative Studies of Electron transport and atrazine binding in thylakoids and PS II particles from spinach. In J. Biggens (Ed.), Progress in Photosynthesis Research (Vol. III, pp. 783-786). Dordrecht:Martinus Nijhoff Publishers

1. The susceptibility of the p- benzoquinone-mediated electron transport and atrazine binding to trypsin and its modification by CaCl2 in thylakoids and PS II membrane fragments


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