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Giovanna Ghirlanda

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School of Molecular Sciences
Professor, Associate Director of Faculty Development
Faculty, TEMPE Campus, Mailcode 1604
Biography: 

Giovanna Ghirlanda is a professor of chemistry at Arizona State University. Her research interests are in chemical biology, protein chemistry, and protein engineering. Professor Ghirlanda received a BSc/MSc degree in Medicinal Chemistry and a PhD in Organic Chemistry from the University of Padova, Italy, with a thesis on the redox catalytic properties of supramolecular metallocomplexes.

Prior to joining ASU, she pursued postdoctoral work at the University of Pennsylvania with Professor William F. DeGrado (now at the University of California San Francisco), specializing on de novo design of peptides and proteins. Her research is in the area of chemical biology and focuses on the design of functional proteins. A major thrust is in the area of sustainable fuel production, designing protein-based hybrid metalloenzymes that catalyze hydrogen production and carbon dioxide reduction in mild conditions. Other projects in the lab include the design of antiviral lectins and work on protein folding in WW domains, in collaboration with Professor Banu Ozkan (Physics, ASU). 

 

CV
Binary Data Curriculum Vitae
Education: 
  • Ph.D. Organic Chemistry, University of Padova, Italy 1996
  • BSc/MSc. Medicinal Chemistry, University of Padova, Italy 1991

 

 

Research Website URL: 
http://www.public.asu.edu/~gghirlan/Giovannalab/Welcome.html
Research Interests: 

My group uses de novo design and protein engineering to prepare artificial model proteins that can perform a desired function. The purpose of this effort is twofold: first, by reproducing the main properties and functions of natural proteins in a model system, one can test iteratively hypotheses on the specific contribution of each amino acid to the protein activity; second, once the key principles are elucidated, it will be possible to design novel proteins with activities beyond those of natural proteins. Methods utilized include computational modeling, solid phase synthesis, molecular biology techniques and enzymatic screening.

Current projects in the lab are divided in three main areas:

Artificial metalloproteins for solar to fuel energy conversion

The conversion of solar energy to usable fuel requires the assembly of a specialized chain of reactions, each typically facilitated by a metallic active site. To obtain the necessary spatial organization, we designed a family of peptides that can self assemble into large complexes functionalized with specialized active sites. We are using a variety of artificial amino acid and natural ligands to engineer [Fe4S4] clusters, diiron clusters, and manganese sites in scaffolding peptides. The ultimate goal is to catalyze biomimetic reactions such as water splitting, CO2reduction, and hydrogen production.

Design of membrane proteins

Membrane proteins function as gateways to the cell, and are crucial in a number of processes related to energy transduction and cell signaling. We are interested in learning how to control protein-protein interactions and cofactor binding in the membrane. To that end we have designed a stable, membrane soluble protein that utilizes a metal cofactor, heme, as active site.

Engineering novel glycan-binding proteins

We are using protein engineering and directed protein evolution to design reagents capable of targeting glycans with high affinity and specificity. The ability of attaching sugars to proteins, called glycosylation, is the most ubiquitous post-translational modification in eukaryotic cells. It is known to have a crucial role in many biological processes, including the immune response, inflammation, and the early stages of bacterial and viral infections. Therefore, the development of specific glycan recognition domains has applications both in basic research and in the diagnosis and therapy of diseases. As part of this effort, we have recently discovered the molecular mechanism of action of a potent anti-HIV protein, cyanovirin, paving the way for the design of improved antiviral proteins.

We are members of the EFRC Center for Bio-Inspired Solar Fuel Production (http://solarfuel.clas.asu.edu/ ) and of the Center for Membrane Proteins in Infectious Diseases (MPID, http://cemilinks.asu.edu/mpid/ )

Publications: 
  • Carla Casadevall, Haojie Zhang, Shojiang Chen, Dayn J. Sommer, Dong-Kyun Seo, Giovanna Ghirlanda.  Photoelectrochemical Water Oxidation by Cobalt Cytochrome C Integrated-ATO Photoanode.  Catalysts (2021).
  • Taisong Zou, Brian W. Woodrum, Nicholas Halloran, Paul Campitelli, Andrey A. Bobkov, Giovanna Ghirlanda, Sefika Banu Ozkan.  Local Interactions That Contribute Minimal Frustration Determine Foldability.  ACS Publications (2021).
  • Rafael Alcala-Torano, Nicholas Halloran, Noah Gwerder, Dayn J. Sommer, Giovanna Ghirlanda.  Light-Driven CO2 Reduction by Co-Cytochrome b562.  Frontiers in Molecular Biosciences (2021).
  • Anindya Roy, Michael David Vaughn, John Tomlin, Garrett J Coffman, Gerdenis Kodis, Chad Simmons, James P Allen, and Giovanna Ghirlanda. Enhanced Photocatalytic Hydrogen Production by Hybrid Streptavidin-Diiron Catalysts. Chemistry-A European Journal (2020).    
  • Maoqing Dong, Kaleeckal G. Harikumar, Sweta R. Raval, Julianna E. Milburn, Carolyn Clark, Rafael Alcala-Torano, Juan C. Mobarec, Christopher A. Reynolds, G. Ghirlanda, Arthur Christopoulos, Denise Wootten, Patrick M. Sexton, Laurence J. Miller.  Rational development of a high-affinity secretin receptor antagonist.  Biochemical Pharmacology (2020).
  • Viren Pattni, Sara M. Vaiana, Giovanna Ghirlanda, Matthias Heyden.  Modeling Liquid-Liquid Phase Separations of Intrinsically Disordered Proteins on the Micrometer-Scale.  Biophysical Journal (2020).
  • Michele Costantino, Prerna Sharma, Sara M. Vaiana, Giovanna Ghirlanda.  Liquid-Liquid Phase Separation of Intrinsically Disordered Proteins for Development of Membraneless Organelles in Synthetic Cells.  Biophysical Journal (2020).
  • Prerna Sharma, Ismail C. Kazan, Sefika B. Ozkan, Giovanna Ghirlanda.  Design of Novel Lectins by Commputer-Aided Directed Evolution.  Protein Science (2019)
  • Arnau Call, Carla Casadevall, Adrian Romero-Rivera, Vlad Martin-Diaconescu, Dayn J. Sommer,  Silvia Osuna, Giovanna Ghirlanda, Julio Lloret-Fillol,  Improved Electro- and Photocatalytic Water Reduction by Confined Cobalt Catalysts in Streptavidin.  ACS Catalysis (2019)
  • Sara H. Mejias, Zahra Bahrami-Dizicheh, Mantas Liutkus, Dayn Joshep Sommer, Andrei Astashkin, Gerdenis Kodis, Giovanna Ghirlanda, Aitziber L. Cortajarena, Repeat proteins as versatile scaffolds for arrays of redox-active FeS clusters.  Chemical Communications (2019)
  • Zhu T., Carrese P. Woodrum BW, Ghirlanda D., and Ozkan SB. Co-evolved positions in early stage of folding determine the foldability of WW domains. (submitted)                
  • Dayn J. Sommer1, Mathieu Walter#, Rafael Alcala-Torano1, Chad K. Park, and Giovanna Ghirlanda*. Rational design of a hexameric protein assembly stabilized by metal chelation. Biopolymers (2018).            
  • Arnau Call, Dayn J. Sommer, Adrian Romero-Rivera, Sílvia Osuna, Giovanna Ghirlanda,* and Julio Lloret-Fillol*. Improved Activity and Stability of an Electro- and Photocatalytic Water Reduction Cobalt Catalyst by Supramolecular Confinement in Streptavidin. Angewandte der Chemie (resubmitted)                   
  • Dizicheh ZB, Halloran N, Asma W, Ghirlanda G. De-Novo Design of Iron Sulfur Proteins. Methods in Enzymology (2017).
  • Olson TL, Espiritu E, Edwardraja S, Canarie E, Flores M, Williams JC, Ghirlanda G, Allen JP. Biochemical and spectroscopic characterization of dinuclear Mn-sites in artificial four-helix bundle proteins. Biochim Biophys Acta. (2017).
  • Woodrum BW., Maxwell J., Babkov A., Hill R.B., O’Keefe B., and Ghirlanda G. A designed dimer of CV-N displays enhanced antiviral activity Viruses (2016).
  • Alcala-Torano R, Sommer DJ, Bahrami Dizicheh Z, Ghirlanda G. Design Strategies for Redox Active Metalloenzymes: Applications in Hydrogen Production. Methods in Enzymology (2016).
  • Sommer DJ, Alcala-Torano R, Dizicheh ZB, Ghirlanda G. Design of Redox-Active Peptides: Towards Functional Materials. Adv Exp Med Biol. (2016).
  • Flores M., Olsen T.L., Wang D., Edwardraja S., Shinde SB., Williams J.C., Ghirlanda G., and Allen J.P. The Copper Environment in Artificial Metalloproteins Probed by EPR Spectroscopy. Journal of Physical Chemistry B (2015).
  • Sizemore S.M., Cope S.M., Roy A., Ghirlanda G. and Vaiana S.M. End-to-end distance and internal dynamics of calcitonin gene related peptide: a comparison with amylin. Biophysical Journal (2015).
  • Zhen Li, Ashini Bolia, Jason D. Maxwell, Andrey A. Bobkov, Giovanna Ghirlanda*, Banu Ozkan, Claudio J. Margulis. A rigid hinge region is necessary for high affinity binding to cyanovirin and associated constructs. Biochemistry (2015).
  • T. L. Olson, E. Espiritu, S. Edwardraja, C. Simmons, J. C. Williams, G. Ghirlanda, and J. P. Allen. Design of dinuclear manganese cofactors for bacterial reaction centers.  Biochem Biophys acta (2015).
  • Sommer DJ, Vaughn MD, Clark BC, Tomlin J, Roy A, Ghirlanda G. Reengineering cyt b562 for hydrogen production: A facile route to artificial hydrogenases. Biochim Biophys Acta. (2015).
  • Sommer DJ, Roy A, Astashkin A, Ghirlanda G. Modulation of cluster incorporation specificity in a de novo iron-sulfur cluster binding peptide. Biopolymers-Special Issue in Honor of Bill DeGrado (2015).
  • A. Bolia, B. W. Woodrum, A. Cereda, S.B. Ozkan, and G. Ghirlanda. A flexible docking scheme efficiently captures the energetics of glycan:cyanovirin binding. Biophysical Journal (2014).
  • Flory J.D., Simmons C., Lin S., Johnson T., Andreoni A., Ghirlanda G., Liu Y., Yan H., and Fromme P. PNA driven engineering (incorporation, assembly, conjugation) of native proteins into DNA nanocages. Journal of the American CHemical Society (2014).
  • Sommer D.J., Roy A., Schmidt R., Brown C., Gust D., Astashkine A., and Ghirlanda G. A De Novo Designed 2[4Fe-4S] Ferredoxin Mimic Mediates Electron Transfer. Journal of the American Chemical Society (2014).
  • Sommer DJ, Vaughn MD, Ghirlanda G. Protein secondary-shell interactions enhance the photoinduced hydrogen production of cobalt protoporphyrin IX. Chemical Communcations (2014).
  • Wang D, Ghirlanda G, Allen JP. Water oxidation by a nickel-glycine catalyst. Journal of the American Chemical SOciety (2014).
  • SM Cope, SM Sizemore, A Roy, G Ghirlanda, SM Vaiana. Structure and Internal Dynamics of Calcitonin Family Peptides: Implications for Amyloid Formation. Biophysical Journal (2014).
  • SM Sizemore, SM Cope, A Roy, G Ghirlanda, SM Vaiana. Solvent Effects on the Structure and Internal Dynamics of Calcitonin Gene-Related Peptide. Biophysical Journal (2014).
  • A Roy, I Sarrou, MD Vaughn, AV Astashkin, G Ghirlanda. De Novo Design of an Artificial Bis [4Fe-4S] Binding Protein. Biochemistry (2013).
  • BW Woodrum, JD Maxwell, A Bolia, SB Ozkan, G Ghirlanda. The antiviral lectin cyanovirin-N: probing multivalency and glycan recognition through experimental and computational approaches. Biochemical Society Transactions (2013).
  • Cope S., Shinde S., Best R., Ghirlanda G., and Vaiana S. Cyclic N-terminal fragment of amylin forms non-amyloid fibers. Biophysical journal (2013).
  • JD Flory, S Shinde, S Lin, Y Liu, H Yan, G Ghirlanda, P Fromme. PNA-Peptide Assembly in a 3D DNA Nanocage at Room Temperature. Journal of the American Chemical Society (2013).
  • M Faiella, A Roy, D Sommer, G Ghirlanda. De novo design of functional proteins: Toward artificial hydrogenases. Peptide Science/Biopolymers (2013).
  • Over 14 chapters with 2-3 authors each. Design of membrane proteins Methods in Molecular Biology series. (2013).
  • Ashini Bolia, Brian Woodrum, Banu Ozkan, Giovanna Ghirlanda. Reengineering the Oligosaccharide Specificity of CVN with BP_Dock. Biophysical Journal (2013).
  • SM Cope, SM Sizemore, A Roy, G Ghirlanda, SM Vaiana. Effect of Prolines on the Intrinsic Stiffness of Islet Amyloid Polypeptide Variants. Biophysical Journal (2013).
  • SM Sizemore, SM Cope, A Roy, G Ghirlanda, SM Vaiana. Electrostatic Interactions Modulate the Structure and Dynamics of Calcitonin Gene-Related Peptide. Biophysical Journal (2013).
  • T Zou, B Woodrum, G Ghirlanda, B Sefika Ozkan. Deciphering the Protein Folding Code through Zipping Steps. Biophysical Journal 104, 398 (2013).
  • Giovanna Ghirlanda. Computational biology: A recipe for ligand-binding proteins. (2013).
  • Guarise C., Ghirlanda G., Prins L.J, and Scrimin P.M. A multivalent HIV-1 fusion inhibitor based on small helical foldamers. Tetrahedron (2012).
  • Roy A., Madden C. and Ghirlanda G. Photo-induced hydrogen production in a helical peptide incorporating a [FeFe] hydrogenase active site mimic. Chemical Communications (2012).
  • Shinde S., Woodrum W, Cordova J.M., Ghirlanda G. "Modulation of Redox Potential in a Minimalist Heme-Binding Membrane Protein. Journal of Biological Inorganic Chemistry (2012).
  • Halsey, C. M., Xiong, J., Oshokoya, O. O., Johnson, J. A., Shinde, S., Beatty, J. T., Ghirlanda, G., Jiji R., and Cooley J.W. Simultaneous Observation of Peptide Backbone Lipid Solvation and a-Helical Structure by Deep-UV Resonance Raman Spectroscopy. ChemBioChem (2011).
  • Ghirlanda G. Design of membrane proteins: towards functional systems",. Current Opinions in Chemical Biology (2009).
  • Liu, Y.; Carroll, J. R.; Holt, L. A.; McMahon, J.a; Giomarelli, B.a; Ghirlanda, G.*. Multivalent Interactions with gp120 Are Required for the Anti-HIV Activity of Cyanovirin. Biopolymers (2009).
  • Bogani F., Spiriti J., vanderWaart A., Ghirlanda G. O-glycosylation and protein stability: experimental and simulation data. Biophysical Chemistry (2008).
  • Fromme, R., Katiliene, Z., Fromme, P. and Ghirlanda, G. Conformational gating of dimannose binding to the antiviral protein cyanovirin revealed by the crystal structure at 1.35 Å resolution. Protein Science (2008).
  • G/ Ghirlanda. News and Views: Old enzymes, New tricks. (2008).
  • J Cordova, P Noack, S Hilcove, J Lear, Giovanna Ghirlanda. Engineering Function in a Membrane Protein: A Heme-Binding Mutant of GpA. JACS (2007).
  • Jeanine Cordova, Pamela Noack, Simon Hilcove, James Lear, Giovanna Ghirlanda. Design of a Functional Membrane Protein by Engineering a Heme-Binding Site in Glycophorin A. Journal of the American Chemical Society (2007).
  • Raimund Fromme, Zivile Katiliene, Barbara Giomarelli, FEDERICA BOGANI, James McMahon, Toshiyuki Mori, Petra Fromme, Petra Fromme, Giovanna Ghirlanda. A Monovalent Mutant of Cyanovirin-N Provides Insight into the Role of Multiple. Biochemistry (2007).
  • Federica Bogani, Elisabeth McConnell, Lokesh Joshi, Yung Chang, Giovanna Ghirlanda. A Designed Miniature Glycoprotein Analog of Gc-MAF Exhibits Native-like Phagocytic Activity. Journal of the American Chemical Society (2006).
  • Bogani, F, McConnelly, E, Joshi, L, Ghirlanda, Giovanna. . Biopolymers (2005).
  • Cordova, J, Noack, P, Hilcove, S, Ghirlanda, Giovanna. . Biopolymers (2005).
  • Giovanna Ghirlanda, Artur Osyczka, Liu Weixia, Michael Antolovich, Kevin Smith, P Dutton, A Wand, William DeGrado. De Novo Design of a D2-Symmetrical Protein that Mimics the Di-Heme 4-Helix Bundle in Cytochrome bc1. Journal of the American Chemical Society (2004).
  • Giovanna Ghirlanda, S Hilcove, R Pidikiti, J Johansson, J Lear, W Degrado, R Eckenhoff. Volatile anesthetic modulation of oligomerization equilibria in a hexameric model peptide. FEBS Lett (2004).
  • Bogani, F, Joshi, L, Edupuganti, V, Ganta, S, Ghirlanda, Giovanna. . Protein Sci (2004).
  • Bogani, F, McConnell, E, Joshi, L, Ghirlanda, Giovanna. Rational design of an immunomodlatory glycoprotein. Protein Science (2004).
  • Hilcove, S, Bennett, I, Ghirlanda, Giovanna. A Novel Membrane Metalloprotein. Protein Science (2004).
  • Giovanna Ghirlanda, J Lear, N Ogihara, D Eisenberg, W DeGrado. An Hierarchic Approach to the Design of an Hexameric Helical Barrels. J. Mol. Biol (2002).
  • B North, C Summa, Giovanna Ghirlanda, W DeGrado. Dn-Symmetrical Tertiary Templates for the Design of Tubular Proteins. J. Mol. Biol (2001).
  • Yi Tang, Giovanna Ghirlanda, N Vahidei, J Kua, D Mainz, W Goddard, W DeGrado, D Tirrell. Stabilization of Leucine Zipper Coiled Coils by Introduction of Trifluoroleucine. Biochemistry (2001).
  • Yi Tang, Giovanna Ghirlanda, W Petka, T Nakajima, W DeGrado, D Tirrell. Fluorinated Coiled-coils Proteins Prepared in vivo Display Enhanced Thermal and Chemical Stability. Angew. Chem (2001).
  • Ogihara, N, Ghirlanda, Giovanna, Bryson, J, Gingery, M, DeGrado, W, Eisenberg, D. Design of three dimensional domain-swapped dimers and fibrous oligomers. (2001).
  • Anindya Roy, Dayn Joseph Sommer, Robert A Schmitz, Andrei Astachkine, Chelsea Brown, Giovanna Ghirlanda. Design of artificial ferredoxin. Photophysics of Bio-Inspired Solar Energy Conversion (0).
  • Ivan Korendovych, Giovanna Ghirlanda.   Editorial overview: The many facets of protein design: from self-assembled materials to vaccines. (2018).
  • T. Zhu, P. Carrese, B.W. Woodrum, Giovanna Ghirlanda, S.B. Ozkan.  Co-evolved positions in early stage of folding determine the foldability of WW domains.
  • Rafael Alcala-Torano, Dayn J. Sommer, Mathieu Walter, Chad K. Park, Giovanna Ghirlanda.*  Rational design of hexameric protein assembly stabilized by metal chelation.  Biopolymers (2018)
  • Arnau Call, Dayn J. Sommer, Adrian Romero-Rivera, Silvia Osuna, Giovanna Ghirlands,* Julio Lloret-Fillol.*   Improved Activity and Stability of an Electro- and Photocatalytic Water Reducation Cobalt Catalyst by Supramolecular Confinement in Streptavidin.  Angewandte der Chemie
  • Anindya Roy, Michael David Vaughn, John Tomlin, Garrett J. Coffman, Gerdenis Kodis, Chad Simmons, James P. Allen, Giovanna Ghirlanda.  Enhanced Photocatalytic Hydrogen Production by Hybrid Streptavidin-Diiron Catalysts.  JACS 
Research Activity: 
Fall 2021
Course NumberCourse Title
CHM 435Medicinal Chemistry
Summer 2021
Course NumberCourse Title
BDE 792Research
Spring 2021
Course NumberCourse Title
CHM 433Advanced Organic Chemistry I
CHM 531Advanced Organic Chemistry I
BDE 792Research
BDE 799Dissertation
Fall 2020
Course NumberCourse Title
CHM 435Medicinal Chemistry
Summer 2020
Course NumberCourse Title
CHM 231Elementary Organic Chemistry
CHM 435Medicinal Chemistry
BDE 792Research
Spring 2020
Course NumberCourse Title
CHM 233General Organic Chemistry I
BDE 792Research
BDE 799Dissertation
Fall 2019
Course NumberCourse Title
CHM 435Medicinal Chemistry
Summer 2019
Course NumberCourse Title
CHM 231Elementary Organic Chemistry
Spring 2019
Course NumberCourse Title
CHM 435Medicinal Chemistry
CHM 591Seminar
BDE 792Research
BDE 799Dissertation
Summer 2018
Course NumberCourse Title
CHM 231Elementary Organic Chemistry
Spring 2018
Course NumberCourse Title
CHM 435Medicinal Chemistry
CHM 535Medicinal Chemistry
CHM 591Seminar
Summer 2017
Course NumberCourse Title
CHM 231Elementary Organic Chemistry
Spring 2017
Course NumberCourse Title
CHM 435Medicinal Chemistry
CHM 535Medicinal Chemistry
CHM 591Seminar
Presentations: 
  • Tien Olson, Selva Edwardraja, Marco Flores, Giovanna Ghirlanda and James P. Allen. Enzymatic activity of a Mn-binding peptide. International Photosynthesis Conference, St Louis (Aug 2014).
  • Ghirlanda G. Protein-based catalysts for hydrogen production. ACS national meeting (Aug 2014).
  • Ghirlanda G. Directed Evolution of Protein-Based Hybrid Catalysts for Hydrogen Production. Frontiers in Biophysics and Chemical Biology, UCSF (Aug 2014).
  • Sommer, Roy, Ghirlanda. Design of artificial metalloproteins. Gordon Conference Solar Fuels, (Feb 2014).
  • Dayn Sommer and Giovanna Ghirlanda. Design of ferredoxin mimics. Gordon Research Seminar Bioinorganic (Feb 2014).
  • Ghirlanda G. Direceted evolution of artificial hydrogenases. Gordon Conference Peptides (Feb 2014).
  • Ghirlanda G. "Directed Evolution of Protein-Based Hybrid Catalysts for Hydrogen Production". Gordon Conference Ppetides (Feb 2014).
  • Ghrlanda g. Design of artificial hydrogenases. Western photosynthesis conference (Jan 2014).
  • Ghirlanda G. session chair. Western photosynthesis conference (Jan 2014).
  • Dayn Sommer, Roy A., Schmidt R., Astashkine A., Ghirlanda G. De novo Design of Metal Binding Peptides as Fuel Generating Catalysts. GRS Bioinorganic (Jan 2014).
  • Ghirlanda G. Design of artificial hydrogenases. Western Photosynthesis Conference (Jan 2014).
  • G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. DOE meeting (Jul 2013).
  • A. Roy, C. MAdden, A. Astashkine, G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. Royal Society Protein Engineering Meeting, Chester, UK (Apr 2013).
  • G. Ghirlanda. Computational analysis and experimental validation of the glycan-protein interaction in Cyanovirin. Royal Society Protein Engineering Meeting, Chester, UK (Apr 2013).
  • G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. Johns Hopkins University (Apr 2013).
  • G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. Seminar, Virginia Tech (Mar 2013).
  • M. Faiella, A. Roy, D. Sommer, and Giovanna Ghirlanda. De novo design of artificial hydrogenases. L'Oreal Women in Science event, Paris, France (Mar 2013).
  • G. Ghirlanda. Design of functional proteins: towards mimics of hydrogenase. Seminar, University of Rochester (Feb 2013).
  • Anindya Roy (presenter), C. Madden, M. Vaughn, A. Astashkine, and G. Ghirlanda. De novo Design and synthesis of a peptide based functional mimic of [FeFe] hydrogenase". Gordon Research Seminar (Feb 2013).
  • Sai Kumar, G. Ghirlanda, and Claudio MArgulis. MD simulations of cyanovirin. Society for glycobiology (Nov 2012).
  • Ghirlanda, G. Design of functional proteins: towards hydrogen production. PEM6- international peptide engineering meeting (Oct 2012).
  • Justin Flory1,2,3, Sandip Shinde2,3, Su Lin3, Hao Yan2,3, Giovanna Ghirlanda2,3, Petra Fromme2,3. Nucleic Acid Driven Peptide Assembly. PEM6 (Oct 2012).
  • Anindya Roy, Chris Madden, Giovanna Ghirlanda. Design and synthesis of a peptide based functional mimic of [FeFe] hydrogenase. PEM6- international peptide engineering meeting (Oct 2012).
  • Anindya Roy, I. Sarrau, A. Astashkine, and G.Ghirlanda. De novo Design and Synthesis of Artificial [4Fe-4S] Binding Peptide Motif. Protein Society meeting (Aug 2012).
  • Woodrum B., Bolia A., Wang X., Oxkan B. and Ghirlanda G. Dissecting the Determinants for Dimmanose Binding in Cyanovirin-­-N. Protein Society meeting (Aug 2012).
  • Ghirlanda, G. Design of Tailor-Made Glycan Recognition Protein Modules based on Cyanovirin. Physics, chemistry, and biology of membrane proteins (May 2012).
  • Matyushev, D; Woodbury N., Beckstein O.,; Fromme P.; Girlanda G. Physics, chemistry, and biology of membrane proteins. Workshop (May 2012).
  • G. Ghirlanda. De novo design of functional protein assemblies: towards hydrogen production. Heraeus Foundation: Harvesting Light (Apr 2012).
  • Ghirlanda G. Design of functional proteins. Colorado School of Mines (Feb 2012).
  • Ghirlanda, G. Design of functional proteins. EFRC review (Feb 2012).
  • Sizemore S.M., Cope S.M, Shinde S, Ghirlanda G and Vaiana S.M. Transient tertiary contact formation in the CGRP neuropeptide revealed by nanosecond laser spectroscopy. Biophysical Society (Feb 2012).
  • Binder J., Shinde S., DeMunari S., Ghirlanda G., and Levitus M. Monitoring Dimerization of GpA Using FRET. Biophysical Society (Feb 2012).
  • Anindya Roy, Chris Madden, Giovanna Ghirlanda. Design and synthesis of a peptide based functional mimic of [FeFe] hydrogenase. Gordon Graduate Seminars- Bioinorganic Chemistry (Feb 2012).
  • Ruben M., Woodrum BW., and Ghirlanda G. Directed evolution of gp120 binding proteins. Society for glycobiology (Nov 2011).
  • Sai Kumar, G. Ghirlanda, and Claudio MArgulis. MD simulations of cyanovirin. Society for glycobiology (Nov 2011).
  • G.Ghirlanda. Design of functional proteins. Marie Curie Conference, Padova, Italy (Sep 2011).
  • G.Ghirlanda. Rational design of antiviral lectins. UCSF (Sep 2011).
  • G.Ghirlanda. Design of model hydrogenases. DOE HUB meeting (Sep 2011).
  • Sandip Shinde, J. Cordova, B. Woodrum, and Giovanna Ghirlanda. Modulation of function in Heme binding membrane protein. American Peptide society annual meeting (Jun 2011).
  • Anindya Roy, I. Sarrau, K. Redding, and G.Ghirlanda. De novo Design and Synthesis of Artificial [4Fe-4S] Binding Peptide Motif. American Peptide society annual meeting (Jun 2011).
  • Mathieu Walther, Anindya Roy, and G.Ghirlanda. Design of multiprotein assemblies. American Peptide society annual meeting (Jun 2011).
  • G.Ghirlanda, A.K.Jones. Design of artificial hydrogenases. DOE EFRC meeting (May 2011).
  • G.Ghirlanda. Design of functional proteins: towards redox catalysis. Rice University (Feb 2011).
  • G.Ghirlanda. Design of functional proteins: antiviral lectins. UT Houston Medical Center (Feb 2011).
  • G.Ghirlanda. Design of functional proteins. University of Miami (Jan 2011).
  • G.Ghirlanda. Artificial proteins for bioenergetic processes. Pacifichem (Dec 2010).
  • G.Ghirlanda. Multivalent interactions are crucial for CV-N activity. • Protein Society Meeting, San Diego (Jul 2010).
  • G.Ghirlanda. Structure-based design of artificial lectins. • NIH Annual AIDS meeting (Jun 2010).
  • G.Ghirlanda. Design of Functional proteins. Seminar at University of Wisconsin-Madison (Apr 2010).
  • G.Ghirlanda. Structure-based design of artificial lectins. Gordon Conference Peptides (Feb 2010).
  • G.Ghirlanda. Structure-based design of artificial lectins. Gordon Conference Peptides (Feb 2010).
  • G.Ghirlanda. Artificial proteins for bioenergetic processes. AIRE workshop (Nov 2009).
  • G.Ghirlanda. Artificial proteins for bioenergetic processes. University of Missouri-Columbia (Oct 2009).
  • G.Ghirlanda. Multivalency effects in antiviral lectins. Johns Hopkins University (Oct 2009).
  • G.Ghirlanda. Design of functional proteins. Wayne State University (Apr 2009).
  • G.Ghirlanda. Design of functional proteins. University of Delaware (Apr 2009).
  • G.Ghirlanda. Design of functional proteins. University of Michigan (Apr 2009).
  • G. Ghirlanda. Modulation of redox potential in model hemoproteins. NSF Workshop on Physical Organic Chemistry (Sep 2008).
  • G. Ghirlanda. Design of functional proteins. University of Arizona (Sep 2008).
  • G. Ghirlanda. Novel Heme-binding proteins. RMACS Salt Lake City (Jun 2008).
  • G.Ghirlanda. Design of functional proteins. SUNY Buffalo (Feb 2008).
  • Ghirlanda, Giovanna. Sweet entanglement. Regional ACS meeting, Tucson, AZ (Oct 2006).
  • Katiliene, Zivile, Bogani, Federica, Giomarelli, Barbara, Mori, Toshi, Fromme, Petra, Fromme, Raimund, Ghirlanda, Giovanna. Design and Crystal Structure of a Monovalent Mutant of CV-N Provides Insight into the Role of Multivalency. Protein Society meeting (Aug 2006).
  • Bogani, F, Chang, Y, Joshi, L, Ghirlanda, Giovanna. A miniature MAF analog with native-like activity. Protein society meeting (Aug 2006).
  • Cordova, J, Noack, P, Ghirlanda, Giovanna. Design of a membrane protein with peroxidase activity. Protein society meeting (Aug 2006).
  • Ghirlanda, Giovanna. Protein Society Meeting (Aug 2006).
  • Ghirlanda, Giovanna. Biopolymers. Gordon conference (Jun 2006).
  • Bogani, F, Chang, Y, Joshi, L, Ghirlanda, Giovanna. A miniature MAF analog with native-like activity. Protein design and function conference (Jul 2005).
  • Cordova, J, Noack, P, Ghirlanda, Giovanna. Design of a membrane protein with peroxidase activity. Protein design and function conference (Jul 2005).
  • Ghirlanda, Giovanna. Metalloprotein and Protein Design (Jul 2005).
  • Ghirlanda, Giovanna. Proteins. Gordon conference (Jun 2005).
  • Ghirlanda, Giovanna. Design of Functional Proteins. (Oct 2004).
  • Ghirlanda, Giovanna. Protein Society meeting (Aug 2004).
  • Ghirlanda, Giovanna. Bioorganic Chemistry. Gordon conference (Jun 2004).
  • Ghirlanda, Giovanna. Design of functional proteins. (Apr 2004).
  • Ghirlanda, Giovanna. Young Scientists Symposium on career choices. European Symposium of the Protein Society (Apr 2003).
  • Ghirlanda, Giovanna. Design of functional membrane proteins.
  • Ghirlanda, Giovanna. NOVEL MEMBRANE PROTEINS FOR REDOX CATALYSIS. Protein Society Eropean Symposium
Service: 
  • Chemical Sciences, reviewer (2014 - Present)
  • Lectures for untenured faculty, panl member (2014 - Present)
  • ChemPhysChem, reviewer (2014 - Present)
  • Peptide Sciences, reviewer (2014 - Present)
  • Protein Science, reviewer (2014 - Present)
  • ACS workshop in Inorganic Chemistry, Organizer (2014 - Present)
  • Graduate program liaison, Committee member (2014 - Present)
  • JACS, reviewer (2014 - Present)
  • Medicinal Chemistry 4+1 program, Faculty coordinator (2014 - Present)
  • Biological Design admission committee, member (2013 - Present)
  • Biomimicry search committee, committee member (2013 - Present)
  • Medicinal BS/MS program, coordinator (2013 - Present)
  • Volkswagen Stiftung, Reviewer (2013 - Present)
  • Arizona Science Outreach Foundation, consultant (2013 - Present)
  • Biological design graduate program, Admission committee member (2013 - Present)
  • JACS, reviewer (2013 - Present)
  • JMB, reviewer (2013 - Present)
  • NSF CHE, reviewer (mail-in) (2013 - Present)
  • Nature, Reviewer (2013 - Present)
  • Nature, Reviewer (2013 - Present)
  • Chemical Biology search committee, Head of search committee (2012 - Present)
  • Chemical Biology search committee, Head of search committee (2012 - Present)
  • Personnel committee, member (elected) (2012 - Present)
  • Personnel committee, member (elected) (2012 - Present)
  • Personnel committee, member (elected) (2012 - Present)
  • Journal of Computational Chemistry, reviewer (2010 - Present)
  • JACS, reviewer (2010 - Present)
  • LA State Commission, reviewer (2008 - Present)
  • Protein Society funding panel, panelist (2008 - Present)
  • NSF, panel member (2008 - Present)
  • NMR search committee, member (2007 - Present)
  • Safety committee, member (2007 - Present)
  • Science is fun, Co-organizer (2005 - Present)
  • Protein Society, Young Scientists committee (2005 - Present)
  • Research Corp, Reviewer (2005 - Present)
  • AZ Biodesign search committee, Member (2003 - Present)
  • Undergraduate Curriculum Committee, Member (2002 - Present)
  • Photosynthesis and Bioenergy, steering committee member (2012 - 2015)
  • Biomimicry search committee, Committee member (2013 - 2015)
  • NSF Chemistry of Life Processes, Panel Member (2014 - 2014)
  • Biophysical Chemistry, reviewer (2012 - 2012)
  • Photosynthesis and Bioenergy, steering committee member (2012 - 2012)
  • Protein Society, Young scientist committee member (2012 - 2012)
  • Photosynthesis and Bioenergy, steering committee member (2011 - 2011)
  • Protein Society, Young scientist committee member (2011 - 2011)
  • undergraduate committee, committee member (2011 - 2011)
  • CUNY, reviewer for tenure case (2011 - 2011)
  • Amino Acids, reviewr (2011 - 2011)
  • NSF, reviewer (2010 - 2010)
  • Undergraduate committee, member (2009 - 2010)
  • CRESMET teacher training initiative, participant (2008 - 2008)
  • NSF, panel member (2007 - 2007)
  • NAture, reviewer (2007 - 2007)
  • Journal of the American Chemical Society, Reviewer (2006 - 2007)
  • Undergraduate Committee, Member (2006 - 2007)
  • Chemistry, NSF, Reviewer (2006 - 2006)
  • Protein Society meeting, San Diego, Judge for students poster award (2006 - 2006)
  • Biochemistry and Molecular Cell Biology, NSF, Reviewer (2006 - 2006)
  • Inorganic Chemistry, Reviewer (2005 - 2005)
  • AZ Biodesign search committee, Member (2004)
  • Gordon Conference, Bioorganic Chemistry, Discussion leader- protein design (2004)
  • Proteins: Structure, Function and Genetics, Reviewer (2003)
  • Young Scientists committee, Protein Society, Organizer and invited speaker (2003)
  • NSF, reviewer (Present)
Expertise Areas: 
Biochemistry
Organic Chemistry
Proteins