As the Vice Dean for Educational Initiatives, Dr. Greenwood works with the academic units at all ASU campuses, including deans, department chairs, faculty and staff to strengthen online programming and drive course development that transforms student learning experiences. In this role, Julie oversees Instructional Design and New Media, Learning Experience and Student Success, Adaptive and Personalized Learning, Assessment and Compliance, and the EdPlus Action Lab, leveraging data analytics and educational technology to improve and equalize degree completion. In addition, she is the EdPlus liaison to the University Innovation Alliance and the APLU Personalized Learning Consortium. Julie received her Ph.D. from the Neuroscience Program at the University of Alabama at Birmingham and is Associate Professor in the School of Mathematical and Natural Sciences in New College at ASU.
Ph.D. The University of Alabama at Birmingham
Targeting Glioblastoma Cell Invasion Using Zebrafish Models for 4D Quantitative Imaging
Glioblastoma is an aggressive primary brain tumor with a 5-year survival rate of less than 5%. The ability of glioblastoma cells to invade surrounding brain tissue presents the primary challenge for the success of focal therapeutic approaches. My research interests focus on glioblastoma cell invasion and mechanisms that slow or stop cancer cell movement. To study the invasion process, fluorescently dyed human glioblastoma cells are injected into the brain of embryonic zebrafish. The zebrafish are translucent at this stage allowing us to image into the brain and monitor glioblastoma cells in action. By altering the expression of proteins in the brain environment, specific proteins that simulate or inhibit glioblastoma cell invasion can be identified with potential to develop new approaches for cancer therapy.
Gamble, J.T., Reed-Harris, Y, Barton, C.L, LaDu, J., Tanguay, R., Greenwood, J.A. (2018) Quantification of Glioblastoma Progression in Zebrafish Xenografts: Adhesion to laminin alpha 5 promotes glioblastoma microtumor formation and inhibits cell invasion. Biochemical and Biophysical Research Communications, https://doi.org/10.1016/j.bbrc.2018.10.076.
Pearce, M., Gamble, J., Kopparapu, P., O’Donnell, E., Mueller, M., Jang, H.S., Greenwood, J.A., Satterthwait, A., Tanguay, R., Zhang, X-K, Kolluri, S. (2018) Induction of apoptosis and suppression of tumor growth by Nur77-derived Bcl-2 converting peptide in chemoresistant lung cancer cells. Oncotarget, in press.
Gamble J, Tanguay R, Greenwood JA. (2017) 4D Quantitative Image Analysis of Cancer Cell Invasion in a Brain Microenvironment Using ImageJ Software. Microsc. Microanal. Aug; 23(SI), 1182-1183.
Wehmas, L.C., Tanguay, R.L., Punnoose, A., Greenwood, J.A. (2016) Developing a novel embryo-larval zebrafish xenograft assay to prioritize human glioblastoma therapeutics. Zebrafish 13, 317-29. PMID: 27158859
Wehmas, L.C., Anders, C., Chess, J., Punnoose, A., Pereira, C.B., Greenwood, J.A., Tanguay, R.L. (2015) Comparative metal oxide nanoparticle toxicity using embryonic zebrafish. Toxicol. Rep. 2, 702-15. PMID: 26029632
Hau, A.M., Greenwood, J.A., Löhr, C.V., Serrill, J.D., Proteau, P.J., Ganley, I.G., McPhail, K.L., and Ishmael, J.E. (2013) Coibamide A Induces mTOR-Independent Autophagy and Cell Death in Human Glioblastoma Cells. PLOS ONE, e65250. PMID: 23762328
Smith, M., Boenzli, M., Hindagolla, V., Ding, J., Miller, J., Hutchison, J., Greenwood, J.A., Abeliovich, H., and Bakalinsky, A. (2013) Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity. Appl. Environ. Microbiol., 79, 728-33. PMID: 23144132
Wang, R., Löhr, C.V., Fischer, K.A., Dashwood, W.M., Greenwood, J.A., Ho, E., Williams, D.E., Ashktorab, H., Dashwood, M.R., and Dashwood, R.H. (2013) Epigenetic inactivation of endothelin-2 and ET-3 in colon cancer. Int. J. Cancer, 132, 1004-12. PMID: 22865632
Lal, S., La Du, Jane, Tanguay, R.L., and Greenwood, J. A. (2012) Calpain 2 is required for the invasion of glioblastoma cells in the zebrafish brain microenvironment. J. Neurosci. Res., 90, 769-81. PMID: 22183788
Ma, L., Greenwood, J.A., and Schachner, M. (2011) CRP1, a protein localized in filopodia of growth cones, is involved in dendritic growth. J. Neurosci. 31, 16781-91.
Jang, H.S., Lal, S., and Greenwood, J.A. (2010) Calpain 2 is required for glioblastoma cell invasion: regulation of matrix metalloproteinase 2. Neurochem. Res. 35, 1796-1804.
Jang, H.S. and Greenwood, J.A. (2009) Gylcine-rich region regulates cysteine-rich protein 1 binding to actin cytoskeleton. Biochem. Biophys. Res. Commun. 380, 484-488.
Sprague, C.R., Fraley, T.S., Jang, H.S., Lal, S., and Greenwood, J.A. (2008) Phosphoinositide binding to the substrate regulates susceptibility to proteolysis by calpain. J. Biol. Chem. 283, 9217-9223.
Full, S.J., Deinzer, M.L., Ho, P.S., and Greenwood, J.A. (2007) Phosphoinositide binding regulates α-actinin CH2 domain structure: Analysis by hydrogen/deuterium exchange mass spectrometry. Protein Science 16, 2597-2604.
Zhang, Y., Vogel, W.K., McCullar, J.S., Greenwood, J.A., and Filtz, T.M. (2006) Phospholipase C-β3 and –β1 form homodimers, but not heterodimers, through catalytic and carboxy-terminal domains. Mol. Pharmacol. 70, 860-868.
Tran, T.C., Singleton, C., Fraley, T.S., and Greenwood, J.A. (2005) Cysteine-rich protein 1 (CRP1) regulates actin filament bundling. BMC Cell Biology, 6 (December), 45.
Figure 6 was chosen as image of the month. Listed as “Highly Accessed” article.
Fraley, T.S., Pereira, C.B., Tran, T.C., Singleton, C., and Greenwood, J.A. (2005) Phosphoinositide binding regulates α-actinin dynamics: mechanism for modulating cytoskeletal remodeling. J. Biol. Chem. 280, 15479-15482.
Corgan, A.M., Singleton, C., Santoso, C.B., and Greenwood, J.A. (2004) Phosphoinositides differentially regulate a-actinin flexibility and function. Biochem. J. 378, 1067-1072.
Fraley, T.S., Tran, T.C., Corgan, A.M., Nash, C.A., Hao, J., Critchley, D.R., and Greenwood, J.A. (2003) Phosphoinositide binding inhibits a-actinin bundling activity. J. Biol. Chem. 278, 24039-24045.
Greenwood, J. A., Theibert, A. B., Prestwich, G. D., and Murphy-Ullrich, J. E. (2000) Restructuring of focal adhesion plaques by PI 3-kinase: regulation by PtdIns (3,4,5)-P3 binding to a-actinin. J. Cell Biol. 150, 627-642.
Greenwood, J. A., and Murphy-Ullrich, J. E. (1998) Signaling of de-adhesion in cellular regulation and motility. Microsc. Res. Tech. 43, 420-432.
Greenwood, J. A., Pallero, M. A., Theibert, A. B., and Murphy-Ullrich, J. E. (1998) Thrombospondin signaling of focal adhesion disassembly requires activation of phosphoinositide 3-kinase. J. Biol. Chem. 273, 1755-1763.
Murphy-Ullrich, J. E., Pallero, M. A., Boerth, N., Greenwood, J. A., Lincoln, T. M., and Cornwell, T. L. (1996) Cyclic GMP-dependent protein kinase is necessary for thrombospondin and tenascin mediated focal adhesion disassembly. J. Cell Sci. 109, 2499-2508.
Johnson, G. V. W., and Greenwood, J. A. (1995) Understanding the hyperphosphorylation of tau in Alzheimer's disease: importance of examining site-specific phosphorylation in non-disease systems. Neurobiol. Aging 16, 371-374.
Greewood, J. A., and Johnson G. V. W. (1995) Localization and in situ phosphorylation state of nuclear tau. Exp. Cell Res. 220, 332-337.
Greenwood, J.A., Scott, C.W, Spreen, R.C., Caputo, C.B., and Johnson, G.V.W. (1994) Casein kinase II preferentially phosphorylates human tau isoforms containing an amino-terminal insert: identification of threonine 39 as the primary phosphate acceptor. J. Biol. Chem. 269, 4373-4380.
Greenwood, J.A., Troncoso, J.C., Costello, A.C., and Johnson, G.V.W. (1993) Phosphorylation modulates calpain-mediated proteolysis and calmodulin-binding of the 200kDa and 160kDa neurofilament proteins. J. Neurochem. 61, 191-199.
Khilko, S., Greenwood, J.A., and Johnson, G.V.W. (1992) Brain casein kinase 2: affinity purification procedure using immobilized polyethylenimine. Protein Expression and Purification 3, 355-361.
Henrikson, K.P., Greenwood, J.A., Pentecost, B.T., Jazin, E.E., and Dickerman, H.W. (1992) Estrogen control of uterine tissue factor messenger ribonucleic acid levels. Endocrinology 130, 2669-2674.
Johnson, G.V.W., Greenwood, J.A., Costello, A.C., and Troncoso, J.C. (1991) The regulatory role of calmodulin in the proteolysis of individual neurofilament proteins by calpain. Neurochem. Res. 16, 869-873.
Henrikson, K.P., Jazin, E.E., Greenwood, J.A., and Dickerman, H.W. (1990) Prothrombin levels are increased in the estrogen-treated immature rat uterus. Endocrinology 126, 167-175.