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Po-Lin Chiu

Assistant Professor
Faculty, TEMPE Campus, Mailcode 1604
Asst Professor
Faculty, TEMPE Campus, Mailcode 1604
Faculty Member
Faculty, TEMPE Campus, Mailcode 1604

Po-Lin Chiu has always been interested in understanding how biological form follows its function. He earned his bachelor's in Pharmacy at National Taiwan University, Taipei, and in addition to the clinical practice, he started to work in the laboratory and study a viral helicase using X-ray crystallography. To expand knowledge, he pursued his master's degree in electrical engineering at National Taiwan University, Taipei, and has the expertise in optics and signal processing. His Ph.D. was then completed in biophysics at the University of California, Davis, focusing on electron microscopy of a bacterial cyclic nucleotide-modulated channel. After graduation, he moved to Harvard Medical School, Boston, as a postdoctoral fellow to study how lipids interact with membrane proteins using cryogenic electron microscopy (cryo-EM). Now at ASU he continues his research investigations on lipid-protein interaction with a focus on the receptor complex of axonal regeneration using the cryo-EM method combined with biophysical and biochemical techniques. 

  • Ph.D. Biophysics, University of California-Davis 2009
  • M.EE. Electrical Engineering, National Taiwan University, Taipei, Taiwan 2001
  • B.Sc. Pharmacy, National Taiwan University, Taipei, Taiwan 1999
Research Interests: 

Our laboratory is interested in membrane protein structures and lipid-protein interaction.  The current focus is to investigate the interaction between each component of the protein complexes, in the context of the membrane bilayer, that inhibit axonal regeneration.  We are also interested in method development in single-particle cryo-electron microscopy (cryo-EM).  


Peer-reviewed papers
29. DeVore, K. and Chiu, P.-L. Probing structural perturbation of biomolecules by extracting cryo-EM data heterogeneity. Biomolecules 12, 628 (2022). 
28. Baker, A.T., Boyd, R.J., Sarkar, D., Crespo, A.T., Chan, C.K., Bates, E., Waraich, K., Vant, J., Wilson, E., Truong, C.D., Lipka-Lloyd, M., Fromme, P., Vermaas, J., Williams, D., Machiesky, L., Heurich, M., Nagalo, B.M., Coughlan, L., Umlauf, S., Chiu, P.-L., Rizkallah, P.J., Cohen, T.S., Parker, A.L., Singharoy, A., and Borad, M.J. ChAdOx1 interacts with CAR and PF4 with implications for thrombosis with thrombocytopenia syndrome. Sci Adv 7 (2021). (doi: 10.1126/sciadv.abl8213) 
27. Truong, C.D., Williams, D., Zhu, M., Wang, J.C.-H. and Chiu, P.-L. Sample preparation using lipid monolayer for electron crystallographic studies. J Vis Exp 177, e63015 (2021). 
26. Zhang, X., Gui, L., Li, S., Nandi, P., Columbres, R.C., Wong, D.E., Moen, D.R., Lin, H.J., #Chiu, P.-L., and #Chou, T.-F. Conserved L464 in p97 D1-D2 linker is critical for p97 cofactor regulated ATPase activity. Biochem J 478, 3185-3204 (2021). (doi: 10.1042/BCJ20210288) (#equal contributions) 
25. Nandi, P., Li, S., Columbres, R.C.A., Wang, F., Williams, D.R., Poh, Y.-P., #Chou, T.-F., and #Chiu, P.-L. Structural and functional analysis of disease-linked p97 ATPase mutant complexes. Int J Mole Sci 22, 8079 (2021). (doi: 10.3390/ijms22158079) (#equal contributions) 
24. Truong, C.D., Craig, T.A., Cui, G., Botuyan, M.V., Serkasevich, R.A., Chan, K.-Y., #Mer, G., #Chiu, P.-L., and #Kumar, R. Cryo-EM reveals conformational flexibility in apo DNA polymerase zeta. J Biol Chem 297, 100912 (2021). (doi: 10.1016/j.jbc.2021.100912) (#equal contributions) 
23. March, K., Venkatraman, K., Truong, C.D., Williams, D., Chiu, P.-L., and Rez, P. Protein secondary structure signatures from energy loss spectra recorded in the electron microscopy. J Microsc 282, 215-223 (2021). 
22. Godeshala, S., Miryala, B., Dutta, S., Christensen, M.D., Nandi, P., Chiu, P.-L., and Rege, K. A library of aminoglycoside-derived lipopolymer nanoparticles for delivery of small molecules and nucleic acids. J Materials Chem B 8, 8558-8572 (2020). 
21. Yang, J.-H., Williams, D., Kandiah, E., #Fromme, P., and #Chiu, P.-L. Structural basis of redox modulation on chloroplast ATP synthase. Commun Biol 3, 482 (2020). (#equal contributions) 
20. Chang, S.L.Y., Reineck, P., Williams, D., Bryant, G., Opletal, G., El-Demrdash, S.A., Chiu, P.-L., Õsawa, E., Barnard, A.S., and Dwyer, C. Dynamic self-assembly of detonation nanodiamond in water. Nanoscale 12, 5363-5367 (2020). 
19. Robertson, K.E., Craciunescu, F.M., Truong, C., Chiu, P.-L., Fromme, P., and Hansen, D.T. Membrane directed expression in Escherichia coli of virulence factors from the Lyme disease agent Borrelia burgdorferi. Sci Rep 9, 1-15 (2019). 
18. Steber, H.S., Gallante, C., Chiu, P.-L., and Mangone, M. The C. elegans 3’UTRome V2: an updated genomic resource to study 3’UTR biology. Genome Res 29, 2104-2116 (2019). 
17. Toporik, H., Li, J., Williams, D., Chiu, P.-L., and Mazor, Y. The structure of the photosystem I IsiA super-complex. Nat Struct Mole Biol 26, 443-449 (2019). 
16. Aryal, R.P., Kwak, P.B., Tamayo, A.G., Chiu, P.-L., Walz, T., and Weitz, C.J. Macromolecular assemblies of the mammalian circadian clock. Mol Cell 67, 770-782 (2017). 
15. *Kalbermatter, D., *Chiu, P.-L., Jeckelmann, J.-M., Ucurum, Z., Walz, T., and Fotiadis, D. Electron crystallography reveals that substrate release from the PTS IIC glucose transporter is coupled to a subtle conformational change. J Struct Biol 199, 39-45 (2017). (*equal contributions)
14. Leksa, N.C., Chiu, P.-L., Bou-Assaf, G.M., Quan, C., Liu, Z., Goodman, A.B., Chambers, M.G., Tsutakawa, S.E., Hammel, M., Peters, R.T., Walz, T., and Kulman, J.D. The structural basis for the functional comparability of factor VIII and the long-acting variant rFVIIIFc. J Thromb Haemost 15, 1167-1179 (2017). 
13. Klara, S.S., Saboe, P.O., Sines, I.T., Babaei, M., Chiu, P.-L., DeZorzi, R., Dayal, K., Walz, T., Kumar, M., and Mauter, M.S. Magnetically directed two-dimensional crystallization of OmpF membrane proteins in block copolymers. J Am Chem Soc 138, 28-31 (2016). 
12. Chiu, P.-L., Li, X., Li, Z., Beckett, B., Brilot, A.F., Grigorieff, N., Agard, D.A., Cheng, Y., and Walz, T. Evaluation of super-resolution performance of the K2 electron counting camera using 2D crystals of aquaporin-0. J Struct Biol 192, 163-173 (2015). 
11. Kalbermatter, D., Jeckelmann, J.-M., Chiu, P.-L., Ucurum, Z., Walz, T., and Fotiadis, D. 2D and 3D crystallization of the wild-type IIC domain of the glucose PTS transporter from Escherichia coli. J Struct Biol 191, 376-380 (2015). 
10. *Chiu, P.-L., *Bou-Assaf, G., Chhabra, E.S., Chambers, M.G., Liu, L., Peters, R., Kulman, J.D., and Walz, T. Mapping the interaction between factor VIII and von Willebrand factor by electron microscopy and mass spectrometry. Blood 126, 935-938 (2015). (*equal contributions)
9. *Hite, R.K., *Chiu, P.-L., Schuller, J., and Walz, T. Effect of lipid head groups on double-layered two-dimensional crystals formed by aquaporin-0. PLoS One 10, e0117371 (2015). (*equal contributions)
8. Kowal, J., Chami, M., Baumgartner, P., Arheit, M., Chiu, P.-L., Rangl, M., Scheuring, S., Nimigean, C.M., and Stahlberg, J. Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1. Nat Commun 5, 3106-3115 (2014). 
7. Hopkins, L.E., Patchin, E.S., Chiu, P.-L., Brandenberger, C., Smiley-Jewell, S., and Pinkerton, K.E. Nose-to-brain transport of aerosolized quantum dots following acute exposure. Nanotoxicol 8, 885-893 (2014). 
6. Evans, J.E., Jungjohann, K.L., Wong, P.C.K., Chiu, P.-L., Dutrow, G.H., Arslan, I., and Browning, N.D. Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy. Micron 43, 1085-1090 (2013). 
5. Chiu, P.-L., Kelly, D.F., and Walz, T. The use of trehalose in the preparation of specimens for electron microscopy. Micron 42, 762-772 (2011). 
4. Paoli, E.-E., Kruse, D.E., Seo, J.W., Zhang, H., Kheirolomoom, A., Watson, K.D., Chiu, P.-L., Stahlberg, H., and Ferrara, K.W. An optical and microPET assessment of thermally sensitive liposome biodistribution in the Met-1 tumor model: importance of formulation. J Control Release 143, 13-22 (2010). 
3. Chiu, P.-L., Pagel, M.D., Evans, J.E., Chou, H.-T., Zeng, X., Gipson, B., Stahlberg, H., and Nimigean, C.M. The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 Å resolution. Structure 15, 1053-1064 (2007). 
2. Renault, L., Chou, H.-T., Chiu, P.-L., Hill, R.M., Zeng, X., Gipson, B., Zhang, Z.Y., Cheng, A., Unger, V., and Stahlberg, H. Milestones in electron crystallography. J Comput Aided Mol Des 20, 519-527 (2006). 
1. Chiu, P.-L., Wu, C.-Y., Taso, J.-H., and Chang, F.-H. Drug delivery through liposome by ultrasonic cavitation. Biomed Eng-App Basis & Comm 13, 47-52 (2001). 

Conference papers
12. Chan, K.-Y., Poh, Y.-P., and Chiu, P.-L. Structural study of regulated intramembrane proteolysis of the p75 neurotrophin receptor by gamma-secretase. Biophys J (2022). 
11. Waheeda, K. and Chiu, P.-L. Complex formation of Rubisco and Rubisco activase. Biophys J (2022). 
10. Chiu, P.-L. Evaluation of hollow-cone electron imaging on biomolecules. Biophys J (2022). 
9. Orjuela, J.D., Chiu, P.-L., Walz, T., de Groot, B.L., and Aponte-Santamaria, C.A. The interplay between cholesterol and aquaporin-0. Biophys J (2022). 
8. Nandi, P., Li, S., Coulmbres, R.C.A., Wang, F., Williams, D.R., Poh, Y.-P., Chou, T.-F., and Chiu, P.-L. Cryo-EM reveals altered domain-domain communications in dementia-mutant R155H human p97/VCP ATPase. Biophys J (2022).  
7. Chiu, P.-L., Yang, J.-H., Williams, D., Kandiah, E., Fromme, P. Redox modulation on chloroplast ATP synthase. Acta Crystallographica Section A. Foundations and Advances (2021). 
6. Nandi, P., Li, S., Columbres, R.C.A., Wang, F., Williams, D.R., Malyutin, A.G., Poh, Y.-P., Chou, T.-F., and Chiu, P.-L. Structural and functional analysis of p47 cofactor binding on the p97 disease mutant. Microsc & Microana (2021). 
5. Nandi, P., Poh, Y.-P., and Chiu, P.-L. Structural studies of the neuronal apoptotic complex of proNGF-p75NTR-sortilin. Biophys J (2021). 
4. Yang, J.-H., Williams, D., Kandiah, E., Fromme, P., and Chiu, P.-L. Redox Modulation on Chloroplast ATP Synthase. Microsc & Microana (2020). doi: 10.1017/S1431927620013458. 
3. Nandi, P., and Chiu, P.-L. Structural studies using cryo-EM to unravel mechanistic details of p47 binding to p97. Biophys J 118, 501a (2020). doi: 10.1016/j.bpj.2019.11.2765. 
2. Luu, D., Chiu, P.-L., and Van Horn, W.D. Isolation of functional temperature activated transmembrane domain of human TRPM8. Biophys J (2020). 
1. Yang, J.-H., Fromme, P., and Chiu, P.-L. Mechanism of light regulation on chloroplast ATP synthase revealed by single-particle cryo-EM. BBA-Bioenergetics 1859, e84-e85 (2018).

Book chapters
1. Chan, K.-Y., Truong, C.D., Poh, Y.-P., and **Chiu, P.-L. Sample preparation and data collection for electron crystallographic studies on membrane protein structures and lipid-protein interaction. Methods in Mole Biol 2215, 247-265 (2021).

Research Activity: 

Assistant Professor, 2016-now.
School of Molecular Sciences and The Biodesign Institute, Arizona State University, Tempe, Arizona.

Postdoctoral Fellow, 2010-2016.
Department of Cell Biology, Harvard Medial School, Boston, Massachusetts.
Laboratory of Thomas Walz.

Postdoctoral Researcher, 2009-2010.
Section of Molecular and Cellular Biology, University of California, Davis, California.
Laboratory of Henning Stahlberg.


Spring 2022
Course NumberCourse Title
BCH 463Biophysical Chemistry
BIO 495Undergraduate Research
Spring 2021
Course NumberCourse Title
BCH 463Biophysical Chemistry
Fall 2020
Course NumberCourse Title
BCH 463Biophysical Chemistry
Spring 2020
Course NumberCourse Title
BCH 463Biophysical Chemistry
Fall 2019
Course NumberCourse Title
BCH 367Elementary Biochemistry Lab
Spring 2019
Course NumberCourse Title
BCH 367Elementary Biochemistry Lab
Fall 2018
Course NumberCourse Title
BCH 494Special Topics
BCH 501Current Topics in Biochemistry
BCH 598Special Topics
Spring 2018
Course NumberCourse Title
BCH 367Elementary Biochemistry Lab
Fall 2017
Course NumberCourse Title
BCH 598Special Topics