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Po-Lin Chiu

Assistant Professor
Faculty, TEMPE Campus, Mailcode 1604
Faculty Member
Faculty, TEMPE Campus, Mailcode 1604

Po-Lin Chiu has always been interested in understanding how biological form follows its function. He earned his bachelor's in Pharmacy at National Taiwan University, Taipei, and in addition to the clinical practice, he started to work in the laboratory and study a viral helicase using X-ray crystallography. To expand knowledge, he pursued his master's degree in electrical engineering at National Taiwan University, Taipei, and has the expertise in optics and signal processing. His Ph.D. was then completed in biophysics at the University of California, Davis, focusing on electron microscopy of a bacterial cyclic nucleotide-modulated channel. After graduation, he moved to Harvard Medical School, Boston, as a postdoctoral fellow to study how lipids interact with membrane proteins using cryogenic electron microscopy (cryo-EM). Now at ASU he continues his research investigations on lipid-protein interaction with a focus on the receptor complex of axonal regeneration using the cryo-EM method combined with biophysical and biochemical techniques. 

  • Ph.D. Biophysics, University of California-Davis 2009
  • M.EE. Electrical Engineering, National Taiwan University, Taipei, Taiwan 2001
  • B.Sc. Pharmacy, National Taiwan University, Taipei, Taiwan 1999
Research Interests: 

Our laboratory is interested in membrane protein structures and lipid-protein interaction.  The current focus is to investigate the interaction between each component of the protein complexes, in the context of the membrane bilayer, that inhibit axonal regeneration.  We are also interested in method development in single-particle cryo-electron microscopy (cryo-EM).  


Zhang, X., Gui, L., Li, S., Nandi, P., Wong, D.E., Moen, D.R., Lin, H.J., Chiu, P.-L. and Chou, T.-F. p97 D1-D2 linker regulates inter-communication between N-domain and D2 ATPase. Biochem J (2021). (accepted)

Nandi, P., Li, S., Columbres, R.C.A., Wang, F., Williams, D.R., Poh, Y.-P., Chou, T.-F. and Chiu, P.-L. Structural and functional analysis of disease-linked p97 mutant complexes. Int J Mole Sci 22:8079 (2021).

Truong, C.D., Craig, T.A., Cui, G., Botuyan, M.V., Serkasevich, R.A., Chan, K.-Y., Mer, G., Chiu, P.-L., and Kumar, R. Cryo-EM reveals conformational flexibility in apo DNA polymerase zeta. J Biol Chem 297:100912 (2021). 

Nandi, P., Poh, Y.-P., and Chiu, P.-L. Structural studies of the neuronal apoptotic complex of the ProNGF-p75NTR-sortilin.  Biophys J (2021). 

Chen, K.-Y., Truong, C.D., Poh, Y.-P., and Chiu, P.-L. Sample preparation and data collection for electron crystallographic studies on membrane protein structures and lipid-protein interaction. Methods Mole Biol 2215:247-265 (2021).

March, K., Venkatraman, K., Truong, C.D., Williams, D., Chiu, P.-L., and Rez, P. 2020. Protein secondary structure signatures from energy loss spectra recorded in the electron microscope. J Microsc 282:215-223 (2021). 

Godeshala, S., Miryala, B., Dutta, S., Christensen, M.D., Nandi, P., Chiu, P.-L., and Rege, K. 2020. A library of aminoglycoside-derived lipopolymer nanoparticles for delivery of small molecules and nucleic acids. J Materials Chem B 8:8558-8572. 

Yang, J.-Y., Williams, D., Kandiah, E., Fromme, P. and Chiu, P.-L. 2020. Structural basis of redox modulation on chloroplast ATP synthase. Commun Biol 3:482. 

Nandi, P. and Chiu, P.-L., 2020. Structural Studies using Cryo-EM to Unravel Mechanistic Details of p47 Binding To p97.  Biophysical J 118: 501A-501A.

Luu, D., Chiu, P.-L., Van Horn, W. D. 2020. Isolation of Functional Temperature Activated Transmembrane Domain of Human TRPM8. Biophysical J 118: 413A-413A.

Chang, S.L.Y., Reineck, P., Williams, D., Bryant, G., Opletal, G., El-Demrdash, S.A., Chiu, P.-L., Õsawa, E., Barnard, A.S., and Dwyer, C. 2020. Dynamic self-assembly of detonation nanodiamond in water. Nanoscale 12:  5363-5367.

Robertson, K.E., Craciunescu, F.M., Truong, C.D., Chiu, P.-L., Fromme, P., and Hansen, D.T. 2019. Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorfeniSci Rep 9:1-15.

Steber, H.S., Gallante, C., Chiu, P.-L., and Mangone, M. 2019. The C. elegans 3’UTRome V2: an updated genomic resource to study 3’UTR biology. Genome Res 29:2104-2116..

Toporik, H., Li, J., Williams, D., Chiu, P.-L., and Mazor, Y. 2019. The structure of the stress-induced photosystem I IsiA super-complex. Nat Struct Mole Biol 26:443-449.

Aryal, R.P.*, Kwak, P.B.*, Tamayo, A.G., Chiu, P.-L., Walz, T., and Weitz, C.J. 2017. Macromolecular assemblies of the mammlian circadian clock. Mol Cell 67:770-782. (*equal contribution). 

Kalbermatter, D.*, Chiu, P.-L.*, Jeckelmann, J.-M., Ucurum, Z., Walz, T., and Fotiadis, D. 2017. Electron crystallography reveals that substrate release from the PTS IIC glucose transporter is coupled to a subtle conformational change. J Struct Biol 199:39-45(*equal contribution)

Leksa, N.C., Chiu, P.-L., Bou-Assaf, G.M., Quan, C., Liu, Z., Goodman, A.B., Chambers, M.G., Tsutakawa, S.E., Hammal, M., Peters, R.T., Walz, T., and Kulman, J.D. 2017. The structural basis for the functional comparability of factor VIII and the long-acting variant rFVIIIFc. J Thromb Haemost 15:1167-1179. 

Klara, S.S., Saboe, P.O., Sines, I.T., Babaei, M., Chiu, P.-L., DeZorzi, R., Dayal, K., Walz, T., Kumar, M., and Mauter, M.S. 2016. Magnetically directed two-dimensional crystallization of OmpF membrane proteins in block copolymers. J Am Chem Soc 138:28-31.

Chiu, P.-L.*, Li, X.*, Li, Z., Beckett, B., Brilot, A.F., Grigorieff, N., Agard, D.A., Cheng, Y., and Walz, T. 2015. Evaluation of super-resolution performance of the K2 electron counting camera using 2D crystals of aquaporin-0. J Struct Biol 192:163-173. (*equal contributions)

Kalbermatter, D., Jeckelmann, J.-M., Chiu, P.-L., Ucurum, Z., Walz, T., and Fotiadis, D. 2015. 2D and 3D crystallization of the wild-type IIC domain of the glucose PTS transporter from Escherichia coli. J Struct Biol 191:376-380.

Chiu, P.-L.*, Bou-Assaf, G.*, Chhabra, E.S., Chambers, M.G., Liu, L., Peters, R., Kulman, J.D., and Walz, T. 2015. Mapping the interaction between factor VIII and von Willebrand factor by electron microscopy and mass spectrometry. Blood 126:935-938. (*equal contributions)

Hite, R.K.*, Chiu, P.-L.*, Schuller, J., and Walz, T. 2015. Effect of lipid head groups on double-layered two-dimensional crystals formed by aquaporin-0. PLoS ONE 10:e0117371. (*equal contributions)

Kowal, J., Chami, M., Baumgartner, P., Arheit, M., Chiu, P.-L., Rangl, M., Scheuring, S., Nimigean, C.M., and Stahlberg, H. 2014. Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1. Nat Commun 5:3106-3115.

Hopkins, L.E., Patchin, E.S., Chiu, P.-L., Brandenberger, C., Smiley-Jewell, S., and Pinkerton, K.E. 2014. Nose-to-brain transport of aerosolized quantum dots following acute exposure. Nanotoxicol 8:885-893.

Evans, J.E., Jungjohann, K.L., Wong, P.C.K., Chiu, P.-L., Dutrow, G.H., Arslan, I., and Browning, N.D. 2013. Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy. Micron 43:1085-1090.

Chiu, P.-L., Kelly, D.F., and Walz, T. 2011. The use of trehalose in the preparation of specimens for electron microscopy. Micron 42:762-772.

Paoli, E.-E., Kruse, D.E., Seo, J.W., Zhang, H., Kheirolomoom, A., Watson, K.D., Chiu, P.-L., Stahlberg, H., and Ferrara, K.W. 2010. An optical and microPET assessment of thermally-sensitive liposome biodistribution in the Met-1 tumor model: importance of formulation. J Control Release 143:13-22.

Chiu, P.-L., Pagel, M.D., Evans, J.E., Chou, H.-T., Zeng, X., Gipson, B., Stahlberg, H., and Nimigean, C.M. 2007. The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 Å resolution. Structure 15:1053-1064.

Renault, L., Chou, H.-T., Chiu, P.-L., Hill, R.M., Zeng, X., Gipson, B., Zhang, Z.Y., Cheng, A., Unger, V., and Stahlberg, H. 2006. Milestones in electron crystallography. J Comput Aided Mol Des 20:519-527.

Chiu, P.-L., Wu, C.-Y., Taso, J.-H., and Chang, F.-H. 2001. Drug delivery through liposome by ultrasonic cavitation. Biomed Eng-App, Basis, & Comm 13:47-52.   

Research Activity: 

Assistant Professor, 2016-now.
School of Molecular Sciences and The Biodesign Institute, Arizona State University, Tempe, Arizona.

Postdoctoral Fellow, 2010-2016.
Department of Cell Biology, Harvard Medial School, Boston, Massachusetts.
Laboratory of Thomas Walz.

Postdoctoral Researcher, 2009-2010.
Section of Molecular and Cellular Biology, University of California, Davis, California.
Laboratory of Henning Stahlberg.


Spring 2021
Course NumberCourse Title
BCH 463Biophysical Chemistry
Fall 2020
Course NumberCourse Title
BCH 463Biophysical Chemistry
Spring 2020
Course NumberCourse Title
BCH 463Biophysical Chemistry
Fall 2019
Course NumberCourse Title
BCH 367Elementary Biochemistry Lab
Spring 2019
Course NumberCourse Title
BCH 367Elementary Biochemistry Lab
Fall 2018
Course NumberCourse Title
BCH 494Special Topics
BCH 501Current Topics in Biochemistry
BCH 598Special Topics
Spring 2018
Course NumberCourse Title
BCH 367Elementary Biochemistry Lab
Fall 2017
Course NumberCourse Title
BCH 598Special Topics