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Alexandra Ros

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Associate Professor
Faculty, TEMPE Campus, Mailcode 1604
Assoc Professor
Faculty, TEMPE Campus, Mailcode 1604
Biography

Alexandra Ros is an associate professor in the School of Molecular Sciences and faculty member of the Center for Applied Structural Discovery (CASD) at the Biodesign Institute. She received her Diploma in chemistry from the Ruprecht-Karls University in Heidelberg, Germany, and her Ph.D. from the Swiss Federal Institute of Technology, Lausanne, Switzerland. Professor Ros has been interested in microfluidic platforms and their analytical applications, since completing her doctorate. She joined the Biophysics and Nanoscience Group at Bielefeld University, Germany, in 2000 where she followed her interests in microfluidics and biophysics during her post doctoral training. Since 2001, she served as principle investigator at Bielefeld University, Germany, on several projects investigating migration mechanisms and single cell analysis in the microfluidic format. In 2007, she finished her Habilitation and received the Venia Legendi in Experimental Physics from Bielefeld University.

Professor Ros joined Arizona State University in 2008 as an assistant professor where she was promoted to associate professor in 2014. In the same year she was appointed faculty member of the Center for Applied Structural Discovery (CASD) at the Biodesign Institute. In 2015-16, she was a visiting scientist at the Georg-August University Goettingen, Germany. She received a National Science Foundation Career Award in 2012, as well as a Fellowship for Experienced Researchers from the Alexander-von-Humboldt Foundation, Germany, in 2015. Her current research interests include migration mechanisms in the micro- and nanoenvironment, single cell analysis, surface design and developing microfluidic tools for crystallography.

Education
  • Ph.D. Ecole Polytechnique Federal de Lausanne 2000
  • Diploma. Chemistry, Ruprecht-Karls University in Heidelberg, Germany 1994
Research Interests

The research in my group focuses on the chemical and physical basis of migration mechanisms for biopolymers and colloids as model systems. Tailored micro- and nanofluidic devices are developed wherein migration processes of biomolecules (e.g. DNA, proteins) are studied by optical single molecule as well as ensemble techniques. A second focus lies on the development of micro- and nanofluidic devices as platforms for novel single cell analysis tools. The ultimate goal consists in elucidating novel bioanalytical and biotechnological tools as well as efficient lab-on-a-chip systems for proteom research and systems nanobiology.

Migration mechanisms of biomolecules are studied via fluorescence video microscopy with subsequent particle tracking and on-chip separation experiments. A major interest lies in the dielectrophoretic behaviour of biomolecules which can be advantageously used in separation experiments. A very important part of our work is dedicated to adequate surface design, providing not only for the control of electrokinetic properties in the micro-and nanodevices but also as an additional design tool for novel migration mechanisms. Soft lithography serves as a primary fabrication method for tailored microdevices, whereas other appropriate micro- and nanofabrication methods find application for specific structuring problems. Our single cell studies focus on biologically relevant questions in organisms such as insect cells and bacteria. Single cell manipulation is developed for reproducible and parallel cell positioning. Our work also includes the integration of ultra sensitive detection methods in the microfluidic chip such as laser induced fluorescence as well as off-chip coupling to sensitive state of the art detection methods.

Publications
  • A. Nakano, J. Luo, A. Ros. Temporal and Spatial Temperature Measurement in Insulator-based Dielectrophoretic Devices. Analytical Chemistry (2014).
  • C. Kupitz, … T.-C. Chao,… A. Ros,… J.C.H. Spence & P. Fromme. Serial Time-resolved Crystallography of Photosystem II Using a Femtosecond X-ray Laser. Nature (2014).
  • J. Luo, B. Abdallah, G. G. Wolken, E. A. Arriaga, A. Ros. Insulator-based dielectrophoresis of mitochondria. Biomicrofluidics (2014).
  • S. Bhattacharya, T.-C. Chao, N. Ariyasinghe, Y. Ruiz, D. Lake, R. Ros, A. Ros. Selective trapping of single mammalian breast cancer cells by insulator-based dielectrophoresis. Analytical and Bioanalytical Chemistry (2014).
  • W. B. Christenson, I. S. Yermolenko, B. Pochberger, F. Camacho-Alanis, A. Ros, T. P. Ugarova, R. Ros. Combined single cell AFM manipulation and TIRFM for probing the molecular stability of multi-layer fibrinogen matrices. Ultramicroscopy (2014).
  • A. Nakano, F. Camacho Alanis, A. Ros. Insulator-based Dielectrophoretic Behavior of ß-Galactosidase under DC and Low Frequency AC Conditions. µTAS Proceedings (2014).
  • B. G. Abdallah, M. Sawtelle, A. Ros. High Throughput Nanoparticle Sorting for Serial Femtosecond Crystallography. µTAS Proceedings (2014).
  • 3. B. G. Abdallah, T.-C. Chao, C. Kupitz, P. Fromme, A. Ros. Photosystem I Nanocrystal Fractionation in a Dielectrophoretic Sorter. ACS Nano (2013).
  • A. Nakano, A. Ros. Protein Dielectrophoresis: Advances, Challenges and Applications. Electrophoresis (2013).
  • B. G. Abdallah, C. Kupitz, P. Fromme, A. Ros. Crystallization of the Large Membrane Protein Complex Photosystem I in a Microfluidic Channel. ACS Nano (2013).
  • J.-U. Kreft, C. Plugge, V. Grimm, C. Prats, J. Leveau, T. Banitz, S. Baines, J. Clark, A. Ros, I. Klapper, C. Topping, T. Field, A. Schuler, E. Litchman, N. Mabrouk, F. Hellweger. Small, simple yet smart: How Observing and Modeling Individual Microbes Advances Science. PNAS (2013).
  • L. Gan, T.-C. Chao, F. Camacho-Alanis, A. Ros. Six Helix Bundle and Triangle DNA Origami Insulator-Based Dielectrophoresis. Analytical Chemistry (2013).
  • R. Martinez-Duarte, F. Camacho-Alanis, P. Renaud, A. Ros. Dielectrophoresis of DNA using 3D Carbon Electrodes. Electrophoresis (2013).
  • Bahige Abdallah, Alexandra Ros. Surface coatings for microfluidic-based medical devices. Microfluidic devices for biomedical applications (2013).
  • M. Yang, T.-C. Chao, R. Nelson, A. Ros. Protein identification and quantification for single cell analysis by coupling a microfluidic platform with MALDI-TOF. 17th µTAS Proceedings (2013).
  • A. Nakano, F. Camacho-Alanis, T.-C. Chao, A. Ros. Tuning Streaming Dielectrophoresis of Proteins. Biomicrofluidics (2012).
  • F. Camacho-Alanis, L. Gan, A. Ros. Transitioning Streaming to Trapping in DC Insulator-based Dielectrophoresis for Biomolecules. Sensors and Actuators B (2012).
  • M. Yang, T.-C. Chao R. Nelson, A. Ros. Direct Detection of Peptides and Proteins on a Microfluidic Platform with MALDI Mass Spectrometry. Analytical and Bioanalytical Chemistry (2012).
  • T.-C. Chao S. Bhattacharya A. Ros. Microfluidic Gel Electrophoresis in the Undergraduate Laboratory Applied to Food Analysis. Journal of Chemical Education (2012).
  • B. Abdallah, T. C. Chao, P. Fromme, A. Ros. Size Based Nanoparticle Separation Using Dielectrophoretic Focusing for Femtosecond Nanocrystallography of Membrane Proteins. Proceedings of 16th µ-Total Analysis Systems Meeting (2012).
  • R. B. Doak, D. P. De Ponte, G. Nelson, F. Camacho-Alanis, A. Ros, J .C. H. Spence, U. Weierstall. Microscopic Linear Liquid Streams in Vacuum: Injection of Solvated Biological Samples into X-Ray Free Electron Lasers. AIP Conference Proceedings (2012).
  • A. Gencoglu, F. Camacho-Alanis, V.T. Nguyen, A. Nakan, A. Ros, A. Minerick. Quantification of pH Gradients and Implications in Insulator-Based Dielectrophoresis of Biomolecules. Electrophoresis (2011).
  • A. Nakano, F. Camacho-Alanis, T.-C. Chao, A. Ros. Immunoglobulin G and Bovine Serum Albumin Streaming Dielectrophoresis in a Microfluidic Device. Electrophoresis (2011).
  • M. Everwand, S. Manchanda, T.-C. Chao, D. Anselmetti, J. Regtmeier, A. Ros. Physisorbed Surface Coatings in Poly(dimethylsiloxane) and Quartz Microfluidic Devices. Analytical and Bioanalytical Chemistry (2011).
  • S. Bhattacharya, T.-C. Chao, A. Ros. Insulator-Based Dielectrophoretic Single Particle and Single Cancer Cell Trapping. Electrophoresis (2011).
  • A. Nakano, F. Camacho-Alanis, T.C. Chao, A. Ros. Systematic Investigation of Insulator-Based Protein Dielectrophoresis under DC Condition. Proceedings of 15th µ-Total Analysis Systems Meeting (2011).
  • A. Ros. Biomolecular Dielectrophoresis. (2011).
  • J. Regtmeier, L. Bogunovic, R. Eichhorn, A. Ros, D. Anselmetti. Dielectrophoretic Trapping and Polarizability of DNA: The Role of Spatial Conformation. Analytical Chemistry (2010).
  • . . Review of: Microchip Methods in Diagnostics (2010).
  • Alexandra Ros, Dominik Greif. Protein Analysis of Single Cells in Microfluidic Format. Single Cell Analysis: Technologies and Applications (2009).
  • Alexandra Ros. Microfluidics in Cell Analysis. Analytical and Bioanalytical Chemistry (2008).
  • Dominik Greif, Lukas Galla, Alexandra Ros, Dario Anselmetti. Single cell analysis in full body quartz glass chips with native UV laser-induced fluorescence detection. Journal of Chromatography A (2008).
  • . . Review of: Microfluidic Technologies for Miniaturized Analysis Systems (2008).
  • Jan Regtmeier, Ralf Eichhorn, Peter Reimann, Alexandra Ros and Dario Anselmetti. DNA DIFFUSION CONTROL. Proceedings of µTAS 2008 (2008).
  • . Microfluidics in Cell Analysis. (2008).
  • Tzu-Chiao Chao and Alexandra Ros. Microfluidic single-cell analysis of intracellular compounds. (2008).
Research Activity
Spring 2017
Course NumberCourse Title
CHM 328Instrumental Analysis Lab
Fall 2016
Course NumberCourse Title
NAN 598Special Topics
CHM 598Special Topics
Spring 2015
Course NumberCourse Title
NAN 598Special Topics
CHM 598Special Topics
Fall 2014
Course NumberCourse Title
CHM 326Advanced Analytical Chem Lab
Spring 2014
Course NumberCourse Title
CHM 328Instrumental Analysis Lab
CHM 501Current Topics in Chemistry
Fall 2013
Course NumberCourse Title
NAN 598Special Topics
CHM 598Special Topics
Presentations
  • B. Abdallah, C. Kupitz, P. Fromme, A. Ros. Fractionation of Nanocrystals for Femtosecond Nanocrystallography of Membrane Proteins. AIChE Annual Meeting (Nov 2013).
  • L. Gan, T.-C. Chao, F. Camacho Alanis, H. Yan and A. Ros. Insulator Based Dielectrophoresis for the Manipulation of DNA Origami. AIChE Annual Meeting (Nov 2013).
  • B. G. Abdallah, C. Kupitz, P. Fromme, A. Ros. Microfluidic Tools to Study Membrane Proteins. X-ray Lasers in Biology, The Royal Society, London, England (Oct 2013).
  • B. G. Abdallah, C. Kupitz, P. Fromme, A. Ros. Microfluidic Tools to Study Membrane Proteins. X-ray Lasers in Biology – Techniques, The Royal Society at Chicheley Hall, Buckinghamshire, England (Oct 2013).
  • J. Luo, B.G. Abdallah, G. Woken, E. Arriaga, A. Ros. Manipulation of Mitochondria by Insulator-based Dielectrophoresis. SciX Conference (Oct 2013).
  • F. Camacho Alanis, A. Nakano, A. Ros. Protein Dielectrophoresis: A Promising Purification Method. SciX Conference (Oct 2013).
  • M. Yang, T.-C. Chao, R. Nelson, A. Ros. Protein identification and quantification for single cell analysis by coupling a microfluidic platform with MALDI-TOF. µTAS conference, Freiburg, Germany (Oct 2013).
  • F. Camacho Alanis, A. Nakano, A. Ros. Protein Dielectrophoresis: A Promising Purification Method. SciX Conference (Oct 2013).
  • J. Luo, B.G. Abdallah, G. Woken, E. Arriaga, A. Ros. Manipulation of Mitochondria by Insulator-based Dielectrophoresis. SciX Conference (Oct 2013).
  • A. Ros. Beyond Miniaturization: Tailored Microfluidic Platforms for Biomolecular Dielectrophoresis and Mass Spectrometry. Invited Department Seminar, Purdue University (Sep 2013).
  • A. Ros. Beyond Miniaturization: Tailored Microenvironments for Bioanalysis. Arizona State University, Tempe (Aug 2013).
  • A. Ros. A Hyphenated Microfluidics and Mass Spectrometric Approach for Single Cell Analysis. Single Cell Genomics and Transcriptomics Asia Congress, Singapore (Jun 2013).
  • A. Ros. Dielectrophoresis Applied to Biomolecule Manipulation and Nanocrystal Sorting. ISCC Conference, Palm Springs (May 2013).
  • A. Ros. Exploiting Microfluidic Environments for Dielectrophoresis of Biomolecules and Nanocrystals. Department Seminar, University of Arizona (May 2013).
  • F. Camacho Alanis, A. Nakano, A. Ros. Dielectrophoresis of proteins in nanostructured microfludic devices. Biophest (Apr 2013).
  • B. Abdallah, A. Ros. Dielectrophoresis of proteins and protein nanocrystals. Biophest (Apr 2013).
  • F. Camacho-Alanis, A. Nakano, A. Ros. Protein Dielectrophoresis under AC and DC Conditions. Microscale Bioseparation (MSB) Conference (Mar 2013).
  • A. Ros. Exploiting Microfluidic Environments for Dielectrophoresis of Biomolecules and Nanocrystals. University of Indiana, Bloomington (Feb 2013).
  • A. Ros. Nanocrystal Sorting in a Microfluidic Device. Southwestern Analytical Professors Meeting, Fort Collins, CO (Jan 2013).
  • F. Camacho Alanis, A. Ros. Insulator-Based Dielectrophoresis Applied to Bovine Serum Albumin and lambda DNA Concentration. Southwestern Analytical Chemistry Professor Meeting 2012 (Jan 2013).
  • A. Ros. Direct coupling of microfluidics and MALDI mass spectrometry for single cell analysis. Southwestern Analytical Chemistry Professor Meeting 2012 (Jan 2013).
  • A. Ros. Surface Coating of Medical Microfluidic Devices. Nanoscience Seminar, Physics Department, ASU (Nov 2012).
  • B. Abdallah, T.-C. Chao, P. Fromme, A. Ros. Dielectrophoretic Sorter for Nanoparticle and Nanocrystal Separation. SciX Conference (Formerly FACSS) (Oct 2012).
  • B. Abdallah, C. Kupitz, P. Fromme, A. Ros. Microfluidic Tools Coupled to Numerical Simulations to Study Membrane Protein Crystallization. SciX Conference (Formerly FACSS) (Oct 2012).
  • M. Yang, T.-C. Chao, R. Nelson, A. Ros. Hyphenation of a Microfluidic Platform with MALDI-TOF Mass Spectrometry for Single Cell Analysis. SciX Conference (Formerly FACSS) (Oct 2012).
  • R. Martinez-Duarte, F. Camacho-Alanis, A. Elkholy, Philippe Renaud, A. Ros. Lambda-DNA Dielectrophoresis in a 3D Carbon-Electrode Micro-Post Device: Theoretical and Experimental Studies. AIChE and American Electrophoresis Society Meeting (Oct 2012).
  • F. Camacho-Alanis, L. Gan, A. Ros. Combining Focused Ion Beam Milling and Optical Lithography to Fabricate Microfluidic Devices for DNA Dielectrophoresis. AIChE and American Electrophoresis Society Meeting (Oct 2012).
  • F. Camacho-Alanis, A. Nakano, T.-C. Chao and A. Ros. Frequency Dependence of Protein Dielectrophoresis Probed with Insulator Based Devices. AIChE and American Electrophoresis Society Meeting (Oct 2012).
  • A. Nakano, F. Camacho-Alanis, T.-C. Chao and A. Ros. AC and DC Protein Streaming and Trapping with Insulator-Based Dielectrophoretic Devices. AIChE and American Electrophoresis Society Meeting (Oct 2012).
  • A. Nakano, K. Bush, A. Ros. Temperature Measurement in a Microfluidic Device for Insulator-Based Dielectrophoretic Applications. AIChE and American Electrophoresis Society Meeting (Oct 2012).
  • S. Bhattacharya, T.-C. Chao, A. Ros. Selective Trapping of Single Human Breast Cancer Cells by Insulator Based Dielectrophoresis in a Microfluidic Device. SciX Conference (Formerly FACSS) (Oct 2012).
  • M. Yang, T.-C. Chao, R. Nelson, A. Ros. Protein affinity capture, digestion and MALDI-TOF detection from a microfluidic platform for Single cell analysis. SciX Conference (Formerly FACSS) (Oct 2012).
  • B. Abdallah, T.-C. Chao, P. Fromme, A. Ros. Size Based Nanoparticle Separation using Dielectrophoretic Focusing for Femtosecond Nanocrystallography of Membrane Proteins. µTAS Conference (Sep 2012).
  • A. Nakano, F. Camacho-Alanis, T.-C. Chao, A. Ros. Dielectrophoretic Protein Manipulation in a Microfluidic Device. ITP 2012-19th Internat. Symposium, Exhibit & Workshops on Electro- and Liquid Phase-separation Techn (Sep 2012).
  • L. Gan, B. Ding, H. Yan, A. Ros. Experimental and Theoretical Study of DNA Origami Dielectrophoresis in a Microfluidic System. ITP 2012-19th Internat. Symposium, Exhibit & Workshops on Electro- and Liquid Phase-separation Techn (Sep 2012).
  • B. Abdallah, C. Kupitz, P. Fromme, A. Ros. Salting-out Crystallization of Photosystem I in a Microfluidic Device. Physics, Chemistry, and Biology of Membrane Proteins Workshop, Arizona State University (May 2012).
  • F. Camacho Alanis, L. Gan, A. Ros. Combining Focused Ion Beam Milling and Optical Lithography to Fabricate Microfluidic Devices for DNA and Protein Dielectrophoresis. Biophest 2012 Conference (Apr 2012).
  • A. Nakano, F. Camacho Alanis, T.-C. Chao, A. Ros. Streaming Dielectrophoresis of Proteins in a Microfluidic Platform. Biophest 2012 Conference (Apr 2012).
  • A. Ros. Two Examples Showing the Potential of Microfluidics for Biomolecule Analysis: Dielectrophoresis and Single Cell Mass Spectrometry. University of California San Diego, Invited Department Colloquium (Apr 2012).
  • A. Ros. Biomolecule Dielectrophoresis. Microscale Bioseparations Conference 2013 (Feb 2012).
  • M. Yang, T.-C. Chiao, R. Nelson, A. Ros. Direct coupling of microfluidics and MALDI mass spectrometry for the detection of peptides and proteins with single cell sensitivity. Microscale Bioseparations Conference 2013 (Feb 2012).
  • A. Ros. Beyond Miniaturization: Tailored Microenvironments for Bioanalytical Applications. IBM Research Laboratories, Rueschlikon, Switzerland (Feb 2012).
  • A. Nakano, F. Camacho-Alanis, T.-C. Chao, A. Ros. Micro- and Nanoenvironments for Dielectrophoretic Manipulation of Proteins. Nanotech Conference, Montreux, Switzerland (Nov 2011).
  • L. Gan, B. Ding, H. Yan, A. Ros. Dielectrophoretic (iDEP) Manipulation of DNA Origami in a Microfluidic System. AIChE and American Electrophoresis Society Meeting (Oct 2011).
  • R. Martinez-Duarte, Philippe Renaud, A. Ros. Concentration of DNA Using 3D Carbon-Electrode Dielectrophoresis. AIChE and American Electrophoresis Society Meeting (Oct 2011).
  • A. Gencoglu, F. Camacho-Alanis, V.T. Nguyen, A. Nakano, A. Ros, A. Minerick. pH Gradient Formation In An Insulator-Based Dielectrophoresis Device Used In Protein Trapping Applications. AIChE and American Electrophoresis Society Meeting (Oct 2011).
  • L. Gan, H. Yan, A. Ros. Dielectrophoretic Manipulation of Naturally Occurring and Origami DNA. FACSS (Federation of Analytical Chemistry and Spectroscopy) Meeting (Oct 2011).
  • A. Nakano, F. Camacho-Alanis, T.-C. Chao, A. Ros. Manipulation of Proteins via Insulator-Based Dielectrophoresis in a Microfluidic Device. FACSS (Federation of Analytical Chemistry and Spectroscopy) Meeting (Oct 2011).
  • A. Ros. Dielectrophoretic Manipulation of Biomolecules in Micro- and Nanofluidic Devices. FACSS (Federation of Analytical Chemistry and Spectroscopy) Meeting (Oct 2011).
  • A. Nakano, F. Camacho-Alanis, T.-C. Chao, A. Ros. Systematic Investigation of Insulator-Based Protein Dielectrophoresis under DC condition. µTAS meeting (Oct 2011).
  • A. Ros. Dielectrophoretic Manipulation of Biomolecules in Micro- and Nanofluidic Devices. Nanoscale Science Seminar, Arizona State University (Sep 2011).
  • A. Ros. Microstructure Induced Biomolecular Separation and Single Cell Analysis in a Lab-on-a-Chip. Department Seminar, Arizona State University (Mar 2011).
  • A. Ros. Single Cell Analysis on Microfluidic Platforms. NIMBIOS Investigative Workshop - Individual-based Ecology of Microbes (Jun 2007).
Service
  • NIH Panel - EBIT, Member (2014 - Present)
  • PlosONE, Academic Editor (2013 - Present)
  • PlosONE, Academic Editor (2013 - Present)
  • Chair, REACH - Program (2013 - Present)
  • Chair, REACH - Program (2013 - Present)