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Wayne Willis

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Associate Professor
Faculty, TEMPE Campus, Mailcode 4501
Biography

Associate Professor, Department of Kinesiology, College of Liberal Arts and Sciences

Office: PEBE 208
Phone: 480-965-7073
Fax: 480-965-8108
waynewillis@asu.edu
Bio

Wayne Willis was hired by the ASU Department of Health and Physical Education in August, 1989 to initiate the study of exercise at the cellular and sub-cellular levels. Prior to his arrival at ASU, Dr. Willis carried out his doctoral research with the Membrane Bioenergetics Group at the University of California, Berkeley, and subsequently spent three years doing postdoctoral work on the effects of dietary iron deficiency on the thermodynamics of mitochondrial oxidative phosphorylation at the University of California Medical Center in San Francisco.

Research

Since the construction of the Exercise Biochemistry Laboratory was completed in 1991, Dr. Willis and his students have studied the basic mechanisms underlying energy transduction in mitochondria isolated from skeletal muscle, liver, and heart. Recent work supported by the National Science Foundation (NSF) has focused on structure/function differences and associated cost/benefit trade-offs in the mitochondria of slow-twitch (type I) versus fast-twitch (type II) skeletal muscle. In the past mainly animal models have been used, but the recent development of the Metabolic Biology Center, along with new micro-techniques for the functional assessment of mitochondria, have made the study of human skeletal muscle mitochondria possible. Over the past five years Dr. Willis, in collaboration with ASU bioengineers and the Neurobiology/Bioengineering Laboratory at Good Samaritan Medical Center, Phoenix, has also been studying fuel selection during human locomotion (primarily walking) in both disabled and able-bodied populations. These experiments have formed the basis of a recent proposal to NSF, addressing the evolutionary implications of walking at a spontaneously chosen speed and the associated skeletal muscle metabolism.

Selected Publications

1. Jackman MR, Willis WT. Characteristics of mitochondria isolated from type I and type IIb skeletal muscle. Am J Physiol 270: C673-C678, 1996.

2. Crowley ME, Willis WT, Matt KS, Donovan CM. A reduced lactate mass explains much of the glycogen sparing associated with training. J Appl Physiol 81: 362-367, 1996.

3. Willis WT, Jackman MR, Bizeau ME, Pagliassotti MJ, Hazel JR. Hyperthermia impairs liver mitochondrial function in vitro. Am J Physiol 278: R1240 - R1246, 2000.

4. Herman R., He J, D'Luzansky S, Willis W, Dilli S. Spinal cord stimulation facilitates functional walking in a chronic, incomplete spinal cord injured. Spinal Cord 40: 65-68, 2002

5. Messer, JI, Jackman MR, Willis WT. Pyruvate and citric acid cycle carbon requirements in isolated skeletal muscle mitochondria. Am J Physiol 286: C565-C572, 2004

6. Ganley KJ, Willis WT, Carhart MR, He J, Herman RM. Epidural spinal cord stimulation improves locomotor performance in low ASIA C wheelchair dependent spinal cord injured individuals: Insights from metabolic response. Top Spinal Cord Inj Rehab 50-63, 2005.

7. Willis WT, Ganley KJ, Herman RM. Fuel oxidation during human walking. Metabolism 54:793-9, 2005

8. Glancy B, Barstow T, Willis W. Linear relation between time constant of oxygen uptake kinetics, total creatine, and mitochondrial content in vitro Am J Physiol (In Review)

Publications
  • Glancy B, Willis W, Chess D, Balaban R. Effect of calcium on the oxidative phosphorylation cascade in skeletal muscle mitochondria. Biochemistry (2013).
  • Kuzmiak S, Glancy B, Sweazea KL, Willis WT. Mitochondrial function in sparrow pectoralis muscle. Journal of Experimental Biology (2012).
  • Lee Y, Min K, Talbert EE, Kavazis AN, Smuder AJ, Willis WT, Powers SK. Exercise protects cardiac mitochondria against ischemia-reperfusion injury. Medine and Science in Sports and Exercise (2012).
  • Ganley KJ, Herman RM, Stock A, Santello, M, and Willis WT. Fuel oxidation at the walk-to-run transition in humans. Metabolism (2011).
  • Lefort N, Glancy B, Bowen B, Willis WT, Bailowitz Z, De Filippis EA, Meyer C, Højlund K, Zhengping Yi Z, and Mandarino LJ. Increased ROS production and lower abundance of complex I subunits and CPT1B protein despite normal mitochondrial respiration in insulin resistant human skeletal muscle. Diabetes (2010).
  • Lefort N, Yi Z, Bowen B, Glancy B, De Filippis EA, Mapes R, Hwang H, Flynn CR, Willis WT, Civitarese A, Højlund K, Mandarino LJ. Proteome profile of functional mitochondria from human skeletal muscle using one-dimensional gel electrophoresis and HPLC-ESI-MS/MS. J Proteomics (2009).
  • K Ganley, Wayne Willis, M Carhart, J He, R Herman. Epidural spinal cord stimulation improves locomotor performance in low ASIA C - wheelchair dependent spinal cord injured individuals: Insights from metabolic response. Topics in Spinal Cord Injury Rehabilitation (2005).
  • Wayne Willis, K Ganley, R Herman. Fuel oxidation during human walking. Metabolism (2005).
  • J Messer, M Jackman, Wayne Willis. Pyruvate and citric acid cycle carbon requirements in isolated skeletal muscle mitochondria. American Journal of Physiology (Cell) (2004).
  • M Carhart, J He, R Herman, S D'Luzansky, Wayne Willis, S Dilli. Epidural Spinal Cord Stimulation facilitates recovery of functional walking following incomplete spinal cord injury. IEEE Trans Neural Syst Rehabil Eng (2004).
  • R Herman, J He, S D'Luzansky, Wayne Willis, S Dilli. Spinal cord stimulation facilitates functional walking in a chronic, incomplete spinal cord injured. Spinal Cord (2002).
  • S Commerford, J Ferniza, M Bizeau, J Thresher, Wayne Willis, M Pagliassotti. Diets enriched in sucrose or fat increase gluconeogenesis and G-6-Pase but not basal glucose production in rats. American Journal of Physiology (2002).
  • J McMillen, C Donovan, J Messer, Wayne Willis. Energetic driving forces are maintained in resting rat skeletal muscle following dietary creatine supplementation. Journal of Applied Physiology (2001).
  • M Bizeau, C Short, J Thresher, S Commerford, Wayne Willis, M Pagliassotti. Increased pyruvate flux capacities account for diet-induced increases in gluconeogenesis in vitro. American Journal of Physiology (2001).
  • Wayne Willis. Thermokinetic control of mitochondrial respiration. Med. Sci. Sports Exerc. (Symposium at the national meeting of ACSM) (2001).
Research Activity
Spring 2015
Course NumberCourse Title
BIO 360Animal Physiology
Fall 2014
Course NumberCourse Title
BIO 189Life Sciences Career Paths
Spring 2014
Course NumberCourse Title
BIO 360Animal Physiology
Spring 2013
Course NumberCourse Title
BIO 494Special Topics
BIO 598Special Topics
Fall 2012
Course NumberCourse Title
BIO 360Animal Physiology
Presentations
  • Willis E, Langlais P, Hudgens J, Mandarino L, Willis W. Simple Michaelis-Menten kinetics describe ADP control of skeletal muscle mitochondrial oxidative phosphorylation. Arizona Physiological Society Annual Meeting, Tucson, AZ (Oct 2014).
  • Kras K, Willis W, Katsanos C. Characteristics of subsarcolemmal vs. intermyofibrillar mitochondria isolated from human skeletal muscle. Arizona Physiological Society Annual Meeting, Tucson, Arizona (Oct 2014).
  • Willis WT. Avian Flight Muscle Mitochondrial Fuel Selection. Seminar, Dept of Exercise Science, Auburn University, Auburn, AL (Sep 2014).
  • Kras K, Willis W, Katsanos C. Using proteolytic enzyme to isolate mitochondria alters mitochondrial function. Experimental Biology Annual Meeting, San Diego, California (Apr 2014).
  • Willis WT. Fuel Selection Implications of Linear Thermodynamic Force-Metabolic Flow Relationships in Mitochondria. Seminar, College of Health Solutions, ASU Phoenix, December 5, 2013 (Dec 2013).
  • Kras K, Willis W, Katsanos C. THE PROTEOLYTIC ENZYME NAGARSE MODIFIES PARAMETERS ASSOCIATED WITH MITOCHONDRIAL FUNCTION OF MECHANICALLY-LIBERATED MITOCHONDRIA. Arizona Physiological Society Annual Meeting (Nov 2013).
  • Willis, WT. Linear Thermodynamic Force-Metabolic Flow Relationships in Mitochondria. Seminar, Dept Kinesiology & Applied Physiology, U of Florida, Gainesville (Oct 2013).
  • CLINTON MIELKE, BANU OZKAN, ANDREW BORDNER, JEREZ TE, WAYNE T. WILLIS, LAWRENCE J. MANDARINO. Molecular Modeling Reveals Acetylation of Adenine Nucleotide Translocase (ANT) 1 Regulates Affinity for ADP. American Diabetes Association Scientific Sessions (Jun 2013).
  • MOULUN LUO, APRIL E. MENGOS, TIANNA M. STUBBLEFIELD, PAUL R. LANGLAIS, WAYNE T. WILLIS, LAWRENCE J. MANDARINO. Clustered Mitochondria Phenotype (Clu)-1 and Lipolysis-Stimulated Lipoprotein Receptor (LSR) Integrate Triglyceride (TG) Uptake and Mitochondrial Content in Liver. American Diabetes Association Scientific Sessions (Jun 2013).
  • Gardner D, Kuzmiak S, Willis W. NAD-Linked Isocitrate Dehydrogenase Activity is Phosphate Dependent in Sparrow Mitochondria. American College of Sports Medicine Annual Meeting (May 2012).
  • Kuzmiak S, Willis W. Electron Conductance in Rat and Sparrow Skeletal Muscle Mitochondrial Electron Transport Chain. American College of Sports Medicine Annual Meeting (May 2012).
  • Kuzmiak S, Gardner D, Pangle P, McInnis H, Willis W. Avian and mammalian skeletal muscle mitochondrial enzyme and electron transport chain activities. Experimental Biology 2012 (Apr 2012).
  • Lefort N, Sharoff C, Kuzmiak S, Yi Z, Langlais P, Cordova J, Mielke C, Ozkan S, Chakkera H, Willis W, Mandarino L. Dysregulation of acetylation of mitochondrial proteins after exercise in insulin resistance. American Diabetes Association Annual Meeting (Jun 2011).
  • Willis W. Mammalian fuel metabolism: Cost-benefit trade-offs. Comparative and Evolutionary Physiology Section of the American Physiological Society (May 2011).
  • Kuzmiak S, Willis W. Pyruvate sparing in avian and mammalian muscle mitochondria. Experimental Biology Meeting (Apr 2011).
  • Kuzmiak S, Schmidt E, Sweazea K, Willis W. Pyruvate sparing by fatty acid and glutamate in sparrow skeletal muscle mitochondria. American Physiological Society/American College of Sports Medicine National Meeting (Oct 2010).
  • Kuzmiak S, Schmidt E, Sweazea K, Willis W. Pyruvate sparing by fatty acid and glutamate in sparrow skeletal muscle mitochondria. Arizona Physiological Society, Midwestern University, Glendale, AZ (Oct 2010).
  • Willis W. Mitochondrial bioenergetics. Welcome to SoLS Seminar, School of Life Sciences, Arizona State University (Aug 2010).
  • Kuzmiak S, Glancy B, Sweazea K, Willis W. Mitochondrial function in sparrow pectoralis muscle. Experimental Biology Annual Meeting (Apr 2010).
  • Willis W, Glancy B, Bowen B, and Barstow T. Kinetics of O2 uptake in isolated mitochondria. American College of Sports Medicine National Meeting, June 2009 (Jun 2009).
  • Exercise Training Protects Cardiac Subsarcolemmal and Interfibrillar Mitochondria against An Ischemia-Reperfusion Injury Youngil Lee, Kisuk Min, Erin E Talbert, Andreas Kavazis, Willis T Wayne, Scott K Powers,. Exercise Training Protects Cardiac Subsarcolemmal and Interfibrillar Mitochondria against An Ischemia-Reperfusion Injury. American Heart Association Annual Meeting (Apr 2009).
  • Brian Glancy, Benjamin P Bowen, Wayne T Willis. A Computer Model of Skeletal Muscle Mitochondrial Metabolism. NIH Meeting on Mitochondrial Metabolism and Disease (Oct 2008).
  • Brian Glancy and Wayne Willis. Superoxide Production by Rat Skeletal Muscle Mitochondria: Influence of High Fat Diet. Experimental Biology Meeting 2008, San Diego, CA (Apr 2008).
  • Morley, E, Glancy, B, Willis, Wayne. Electron transport in uncoupled mitochondrial conforms to Ohm's Law. Southwest American College of Sports Medicine Annual Meeting (Nov 2006).
  • Willis, Wayne, Herman, R. Motor and metabolic control: Part of the same network?. Networks in Motion Symposium, Wenner-Gren Foundation, Stockholm (Sep 2006).
  • Ganley, K, Herman, R, Stock, A, Willis, Wayne. Fuel oxidation at the walk-to-run transition. Experimental Biology Annual Meeting, San Francisco, CA (Apr 2006).
  • Glancy, B, Barstow, T, Willis, Wayne. Linear Relation Between Time Constant of O2 Uptake Kinetics and Total Creatine In Vitro. Experimental Biology Annual Meeting, San Francisco, CA (Apr 2006).
  • Glancy, B, Barstow, T, Willis, Wayne. TOTAL CREATINE PROFOUNDLY INFLUENCES OXYGEN CONSUMPTION RATE KINETICS OF ISOLATED SKELETAL MUSCLE MITOCHONDRIA. American College of Sports Medicine Annual Meeting, Southwestern Region (Nov 2005).
  • Willis, Wayne. Mitochondrial Metabolism (Chair of Free Communication Session). American College of Sports Medicine Annual Meeting (Jun 2005).
  • Ganley, K, Herman, R, Willis, Wayne. Perception of effort tracks carbohydrate oxidation rate during walking. Experimental Biology Meeting, San Diego, California (Apr 2005).
  • Willis, Wayne. Kinetics of skeletal muscle oxygen uptake. Annual Meeting of the Southwest Chapter of the American College of Sports Medicine (Oct 2004).
  • Willis, Wayne, Messer, J. Kinetics of mitochondrial oxidative phosphorylation. Annual Meeting of the Southwest Chapter of American College of Sports Medicine (Oct 2004).
  • Willis, Wayne, Stadler, M, Thresher, J, Herman, R. Is fuel selection a determinant of walking speed?. National Experimental Biology Meeting (Apr 2002).
  • Willis, Wayne. Influence of Two Electrical Stimulation (STIM) Modes on Walking Metabolism and Performance in a Spin. Experimental Biology National Meeting (FASEB)
  • Willis, Wayne. Metabolic and performance effects of electrical stimulation of the spinal cord and peripheral nerve. Society for Neuroscience National Meeting
  • Willis, Wayne. Vmax of Mitochondrial Electron Shuttles in Rat Skeletal Muscle and Liver. American College of Sports Medicine National Meeting
Service
  • Graduate Program Curriculum Development, Chair (2014 - Present)
  • Facilities and Space Committee, Center for Metabolic and Vascular Biology, ASU/Mayo Clinic, Chair (2013 - Present)
  • Facilities and Space Committee, Center for Metabolic and Vascular Biology, ASU/Mayo Clinic, Chair (2013 - Present)
  • Mouse Metabolic Phenotyping Laboratory Committee, Member (2013 - Present)
  • Mouse Metabolic Phenotyping Laboratory Committee, Member (2013 - Present)
  • Cell Metabolism, Ad-Hoc Reviewer (2014 - 2014)
  • Diabetes, Ad Hoc Reviewer (2014 - 2014)
  • American Journal of Physiology - Regulatory, Ad Hoc Reviewer (2014 - 2014)
  • Journal of Experimental Biology, Ad Hoc Reviewer (2014 - 2014)
  • Journal of Physiology (London), Ad-Hoc Reviewer (2014 - 2014)
  • Diabetes, Ad Hoc Reviewer for both manuscripts and grant proposals (2013 - 2013)
  • American Journal of Physiology - Regulatory, Ad Hoc Reviewer (2013 - 2013)
  • Journal of Experimental Biology, Ad Hoc Reviewer (2013 - 2013)
  • Journal of Physiology (London), Ad-Hoc Reviewer (2013 - 2013)
  • Medicine and Science in Sports and Exercise, Ad-Hoc Reviewer (2013 - 2013)
  • Metabolism, Ad-Hoc Reviewer (2013 - 2013)
  • Comprehensive Physiology, Ad-Hoc Reviewer (2012 - 2012)
  • American Journal of Physiology - Regulatory, Ad Hoc Reviewer (2012 - 2012)
  • Comparative Biochemistry and Physiology, Ad Hoc Reviewer (2012 - 2012)
  • Journal of Applied Physiology, Ad Hoc Reviewer (2012 - 2012)
  • Journal of Experimental Biology, Ad Hoc Reviewer (2012 - 2012)
  • Journal of Physiology (London), Ad-Hoc Reviewer (2012 - 2012)
  • Medicine and Science in Sports and Exercise, Ad-Hoc Reviewer (2012 - 2012)
  • Metabolism, Ad-Hoc Reviewer (2012 - 2012)
  • American Journal of Physiology - Regulatory, Member of Editorial Board (2009 - 2011)
  • Comprehensive Physiology, Ad-Hoc Reviewer (2010 - 2010)
  • Journal of Experimental Biology, Ad Hoc Reviewer (2010 - 2010)
  • Journal of Physiology (London), Ad-Hoc Reviewer (2010 - 2010)
  • Metabolism, Ad-Hoc Reviewer (2010 - 2010)
  • American Journal of Physiology (Regulatory), Editorial Board Member (2008 - 2010)
  • Committee for Undergraduate Core Course Online Laboratories, Committee Member (2009 - 2009)
  • American Journal of Physiology, Ad-Hoc Reviewer (2009 - 2009)
  • Director of Exercise Biochemistry Laboratory, Research Supervisor (2009 - 2009)
  • Journal of Experimental Biology, Ad Hoc Reviewer (2009 - 2009)
  • Journal of Physiology (London), Ad-Hoc Reviewer (2009 - 2009)
  • Metabolism, Ad-Hoc Reviewer (2009 - 2009)
  • Revenue generation for ASU, Summer school instructor (2008 - 2009)
  • Special Committee to evaluate lecturers, Chair (2008 - 2008)
  • Workshop on PowerPoint, Instructor (2008 - 2008)
  • Equipment Acquisition, Liaison between Good Samaritan Hospital, Phoenix and ASU (2008 - 2008)
  • American Journal of Physiology, Ad-Hoc Reviewer (2008 - 2008)
  • Journal of Experimental Biology, Ad Hoc Reviewer (2008 - 2008)
  • Journal of Physiology (London), Ad-Hoc Reviewer (2008 - 2008)
  • Letters of recommendation, Faculy Reference (2008 - 2008)
  • Metabolism, Ad-Hoc Reviewer (2008 - 2008)
  • Department Personnel Committee, Chair (2006 - 2008)
  • Masters Committee, Director (2004 - 2008)
  • Search Committee for Assoc Prof in Metabolic Physiology, Chair (2007 - 2008)
  • Search Committee - Assistant Professor in Metabolic Physiology, Chair (2006 - 2007)
  • Letters of Reference, Faculty Reference for students applying to graduate or professional training programs (2006 - 2007)
  • American Journal of Physiology, Ad-Hoc Reviewer (2006 - 2006)
  • Graduate Program Executive Committee, Member (2006 - 2006)
  • Journal of Physiology, Ad-Hoc Reviewer (2006 - 2006)
  • Medicine and Science in Sports and Exercise, Ad-Hoc Reviewer (2006 - 2006)
  • Personnel Committee, Member (2006 - 2006)
  • Probation and Tenure Committee, Member (2006 - 2006)
  • Masters Graduate Program, Director (2004 - 2006)
  • Metabolism, Ad-Hoc Reviewer (2006 - 2006)
  • Journal of Experimental Biology, Ad Hoc Reviewer (2006 - 2006)
  • Search Committee - Assoc Professor in Metabolic Physiology, Chair (2005 - 2006)
  • Bariatric Surgery Program at Good Samaritan Medical Center, Phoenix, Designer/Instructor of Physical Activity Education Unit for Bariatric Patients (2006 - 2006)
  • Research Development - Neurobiology & Bioengineering Laboratory, Good Samaritan Medical Center, member (2005 - 2005)
  • Personnel Committee, Chair (2005 - 2005)
  • ESRI Technician Search Committee, Member (2004 - 2004)
  • Experimental Gerontology, Ad-Hoc Reviewer (2004 - 2004)
  • American Journal of Physiology, Ad-Hoc Reviewer (2004)
  • Journal of Physiology, Ad-Hoc Reviewer (2004)
  • Medicine and Science in Sports and Exercise, Ad-Hoc Reviewer (2004)
  • Probation and Tenure Committee, Member (2004)
  • Search Committee: Department Chair, Provided assistance (2004)
  • Search Committee - Asst. Professor in Motor Control, Member (2003 - 2004)
  • Science Fair - Kiva Elementary School, Scottsdale, Judge (2004 - 2004)
  • Journal of Applied Physiology, Ad-Hoc Reviewer (2003)
  • Kiva Elementary School Science Fair, Scottsdale, Science Fair Judge (2003)
  • Personnel Committee, Chair (2003)
  • Search Committee - Motor Control Faculty Position, Member (2003)